Dabrowiak J C, Skorobogaty A, Rich N, Vary C P, Vournakis J N
Nucleic Acids Res. 1986 Jan 10;14(1):489-99. doi: 10.1093/nar/14.1.489.
A system comprised of a linear scanning microdensitometer interfaced to a personal computer has been developed to facilitate analysis of ligand-DNA footprinting autoradiograms. The system, which can be used to record density and sequence information from autoradiographic films, enables the user to relate the area under an autoradiographic band to the concentration of radiolabeled molecules present in the electrophoresis gel. This report describes the computer program which performs the calculations and discusses the ability of the system to accurately determine oligonucleotide concentration, as a function of band separation, photographic response, and the computational algorithm used to calculate band areas.
已开发出一种由与个人计算机相连的线性扫描显微密度计组成的系统，以促进对配体 - DNA足迹放射自显影片的分析。该系统可用于记录放射自显影片的密度和序列信息，使用户能够将放射自显影条带下方的面积与电泳凝胶中存在的放射性标记分子的浓度联系起来。本报告描述了执行计算的计算机程序，并讨论了该系统根据条带分离、感光响应以及用于计算条带面积的计算算法准确测定寡核苷酸浓度的能力。
