Department of Prosthodontics, SRM Dental College, Chennai, Tamil Nadu, India.
J Indian Prosthodont Soc. 2023 Oct-Dec;23(4):384-390. doi: 10.4103/jips.jips_378_23.
The aim was to evaluate and compare the cell viability, tear bond strength, and shore hardness of MDX4-4210 silicone reinforced with titanium dioxide and silver nanoparticles in 1%, 2%, and 3%.
The study design involves in vitro comparative study.
MDX4-4210 silicone incorporated with 1%, 2%, and 3% by weight of silver and titanium dioxide nanoparticles. A total of 112 specimens were prepared and grouped into 7, with 16 specimens in each group. Group 1 - control, Groups 2, 3, and 4 were 1%, 2%, and 3% silver, respectively. Groups 5, 6, and 7 were 1%, 2%, and 3% titanium dioxide nanoparticles, respectively. Cell viability was tested by MTT ASSAY on MG63 cell lines, tear bond strength was tested by peeling force in universal testing machine, and Shore A hardness was tested in durometer.
The cell viability values were statistically analysed using one-way analysis of variance, and Tukey honestly significant difference test, tear bond strength and shore hardness values were analysed using Mann-Whitney test.
Based on the MTT ASSAY test, 1% silver nanoparticles incorporated MDX4-4210 silicone showed maximum cell viability of 42.10%, whereas minimum cell viability was 18.06% for 3% of titanium dioxide-reinforced silicone. The mean value of tear bond strength of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 62.81 ± 3.637 N/m and 59.69 ± 5.313 N/m and the mean value of shore hardness of room temperature vulcanized of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 38.06 ± 1.237 and 36.75 ± 1.291.
Cell viability of 1% silver nanoparticles reinforced MDX4-4210 silicone was higher in comparison to the other groups, and tear bond strength and shore hardness were significantly higher in 1% silver nanoparticles reinforced silicone compared to 1% titanium nanoparticles reinforced MDX4-4210 silicone.
评估和比较 1%、2%和 3%浓度的二氧化钛和银纳米颗粒增强 MDX4-4210 硅酮的细胞活力、泪液结合强度和肖氏 A 硬度。
本研究设计为体外比较研究。
将 MDX4-4210 硅酮与 1%、2%和 3%重量比的银和二氧化钛纳米颗粒混合。共制备 112 个样本，并分为 7 组，每组 16 个样本。第 1 组为对照组，第 2、3 和 4 组分别为 1%、2%和 3%的银。第 5、6 和 7 组分别为 1%、2%和 3%的二氧化钛纳米颗粒。通过 MG63 细胞系的 MTT 测定法测试细胞活力，通过万能试验机测试剥离力测试泪液结合强度，通过硬度计测试肖氏 A 硬度。
使用单因素方差分析和 Tukey Honestly 显著性差异检验对细胞活力值进行统计学分析，使用 Mann-Whitney 检验对泪液结合强度和肖氏 A 硬度值进行分析。
根据 MTT 测定法，1%银纳米颗粒增强的 MDX4-4210 硅酮显示出 42.10%的最大细胞活力，而 3%二氧化钛增强硅酮的最小细胞活力为 18.06%。1%银和 1%二氧化钛纳米颗粒增强室温硫化颌面硅酮的平均泪液结合强度分别为 62.81±3.637N/m 和 59.69±5.313N/m，1%银和 1%二氧化钛纳米颗粒增强室温硫化颌面硅酮的平均肖氏 A 硬度分别为 38.06±1.237 和 36.75±1.291。
与其他组相比，1%银纳米颗粒增强 MDX4-4210 硅酮的细胞活力更高，与 1%钛纳米颗粒增强 MDX4-4210 硅酮相比，1%银纳米颗粒增强硅酮的泪液结合强度和肖氏 A 硬度显著更高。
