Kazakov O V, Poveshchenko A F, Kabakov A V
Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2023 Sep;175(5):681-684. doi: 10.1007/s10517-023-05925-9. Epub 2023 Oct 20.
The relationship between the expression levels of microRNAs (miR-21, miR-221, miR-222, and miR-429) and morphometric parameters of the thymus was studied in female Wistar rats with breast cancer receiving and not receiving chemotherapy. In breast cancer, the following positive relationships were revealed: miR-221 in the subcapsular zone of the cortical substance with medium lymphocytes, miR-221 in the central part of the cortical substance with immunoblasts and macrophages, miR-429 in the subcapsular zone of the cortical substance with mitotically dividing cells, miR-429 in the central part of the medullary substance with macrophages, and miR-21 with medium lymphocytes of the thymus. In the subcapsular zone of the cortical substance, proliferative activity and the number of cells with pyknotic nuclei were increased. After chemotherapy, the relationship of microRNAs in the lymph with transformations in the thymus can indicate destructive processes in the breast tumor in comparison with breast cancer without treatment and indicate strengthening of the antitumor immunity, a decrease in the number of dying cells, and lymphoid depletion of the morphofunctional zones of the thymic parenchyma. Positive relationships of miR-21 with medium and small lymphocytes, miR-221 and miR-429 with epithelial cells, miR-222 with macrophages in the central part of the cortex, and miR-222 with macrophages in the central part of the medulla were found.
在接受和未接受化疗的乳腺癌雌性Wistar大鼠中，研究了微小RNA（miR-21、miR-221、miR-222和miR-429）表达水平与胸腺形态学参数之间的关系。在乳腺癌中，发现了以下正相关关系：皮质物质被膜下区的miR-221与中等淋巴细胞、皮质物质中部的miR-221与免疫母细胞和巨噬细胞、皮质物质被膜下区的miR-429与有丝分裂细胞、髓质物质中部的miR-429与巨噬细胞以及miR-21与胸腺的中等淋巴细胞。在皮质物质被膜下区，增殖活性和核固缩细胞数量增加。化疗后，与未治疗的乳腺癌相比，淋巴细胞中微小RNA的关系以及胸腺中的变化可表明乳腺肿瘤中的破坏过程，并表明抗肿瘤免疫力增强、死亡细胞数量减少以及胸腺实质形态功能区的淋巴细胞耗竭。发现miR-21与中小淋巴细胞、miR-221和miR-429与上皮细胞、皮质中部的miR-222与巨噬细胞以及髓质中部的miR-222与巨噬细胞呈正相关。
