Huang Shiting, Mi Xue, Ren Tongwei, Hong Daling, Qin Qiuying, Long Meijing, Qin Yifeng, Chen Ying, Wei Zuzhang, Huang Weijian, Ouyang Kang
Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530005, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, 530005, China.
Virology. 2023 Nov;588:109899. doi: 10.1016/j.virol.2023.109899. Epub 2023 Oct 13.
Porcine enterovirus G (EV-G) is endogenous to most pig farming countries worldwide. Reports that a papain-like protease (PLP) gene has been naturally inserted into the 2C/3A junction region of the EV-G genome, has increased the potential public health threats from this virus. We constructed a full-length infectious cDNA clone of EV-G, CH/17GXQZ/2017, in order to determine the packaging capacity at the 2C/3A insertion site. Subsequently, recombinants viruses containing the coding tags, GFP, iLOV and His at the 2C/3A junction region, were synthesized. The infectious virus was successfully rescued only with the insertion of the His-tag, which displayed similar virological and molecular properties to its parental strain. This study determined the packaging capacity of the 2C/3A insertion site, and it provides a practical tool for studying the functions and pathogenic mechanisms of EV-G in pigs.
猪肠道病毒G（EV-G）在全球大多数养猪国家都有本土病例。有报道称，一种类木瓜蛋白酶（PLP）基因已自然插入到EV-G基因组的2C/3A连接区域，这增加了该病毒对公众健康的潜在威胁。我们构建了EV-G的全长感染性cDNA克隆CH/17GXQZ/2017，以确定2C/3A插入位点的包装能力。随后，合成了在2C/3A连接区域含有编码标签GFP、iLOV和His的重组病毒。仅在插入His标签后成功拯救出感染性病毒，其病毒学和分子特性与其亲本毒株相似。本研究确定了2C/3A插入位点的包装能力，并为研究EV-G在猪体内的功能和致病机制提供了实用工具。
