Tsuchiaka Shinobu, Naoi Yuki, Imai Ryo, Masuda Tsuneyuki, Ito Mika, Akagami Masataka, Ouchi Yoshinao, Ishii Kazuo, Sakaguchi Shoichi, Omatsu Tsutomu, Katayama Yukie, Oba Mami, Shirai Junsuke, Satani Yuki, Takashima Yasuhiro, Taniguchi Yuji, Takasu Masaki, Madarame Hiroo, Sunaga Fujiko, Aoki Hiroshi, Makino Shinji, Mizutani Tetsuya, Nagai Makoto
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, Japan.
PLoS One. 2018 Jan 11;13(1):e0190819. doi: 10.1371/journal.pone.0190819. eCollection 2018.
To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C-3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.
为研究日本猪群中肠道病毒G（EV-G）的遗传多样性，对2014年至2016年间采集的有或无腹泻症状猪的粪便样本进行了宏基因组测序。获得了59条长度大于5000个核苷酸的EV-G序列。通过完整的VP1序列分析，日本的EV-G分离株被分为G1（17株）、G2（4株）、G3（22株）、G4（2株）、G6（2株）、G9（6株）、G10（5株）和一个新基因型（1株）。值得注意的是，在腹泻（23.5%；4株）或正常（76.5%；13株）粪便样本中鉴定出的16株G1和1株G2毒株，在2C-3A连接位点具有一种类木瓜蛋白酶半胱氨酸蛋白酶（PL-CP）序列，该序列最近在美国和比利时的EV-G基因组中被发现。本文首次报道了携带PL-CP的病毒在EV-G群体中的高流行率。此外，在EV-G毒株中发现了可能的基因型间和基因型内重组事件，包括G1-PL-CP毒株。我们的研究结果可能会促进对EV-G分子流行病学和遗传进化的理解。
