Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi, 502285, Telangana, India.
Department of Electrical Engineering, Indian Institute of Technology Hyderabad, Kandi, 502285, Telangana, India.
Nanotechnology. 2023 Nov 20;35(6). doi: 10.1088/1361-6528/ad0593.
Fibrinogen, a circulating glycoprotein in the blood, is a potential biomarker of various health conditions. This work reports a flexible electrochemical sensor based on Ni-Fe layered double hydroxide (Ni-Fe LDH) coated on Nickel foam (Ni-Fe LDH/NF) to detect fibrinogen in simulated human body fluid (or blood plasma). The nanoflakes like morphology and hexagonal crystal structure of LDH, synthesized via urea hydrolysis assisted precipitation technique, are revealed by scanning electron microscopy (SEM) and powder x-ray diffraction (PXRD) techniques, respectively. The fabricated sensor exhibits linearity in a wide dynamic range covering the physiological concentration, from 1 ng mlto 10 mg ml, with a sensitivity of 0.0914 mA (ng/ml)(cm). This LDH-based sensor is found to have a limit of detection (LOD) of 0.097 ng mland a limit of quantification (LOQ) of 0.294 ng ml(S/N = 3.3). The higher selectivity of the sensor towards fibrinogen protein is verified in the presence of various interfering analytes such as dopamine, epinephrine, serotonin, glucose, potassium, chloride, and magnesium ions. The sensor is successful in the trace-level detection of fibrinogen in simulated body fluid with excellent recovery percentages ranging from 99.5% to 102.5%, proving the synergetic combination of 2D Ni-Fe layered double hydroxide and 3D nickel foam as a promising platform for electrochemical sensing that has immense potential in clinical applications.
纤维蛋白原是血液中的一种循环糖蛋白，是各种健康状况的潜在生物标志物。本工作报道了一种基于 Ni-Fe 层状双氢氧化物（Ni-Fe LDH）涂覆在泡沫镍（Ni-Fe LDH/NF）上的灵活电化学传感器，用于检测模拟人体液（或血浆）中的纤维蛋白原。通过尿素水解辅助沉淀技术合成的 LDH 的纳米片状形貌和六方晶体结构分别通过扫描电子显微镜（SEM）和粉末 X 射线衍射（PXRD）技术揭示。所制备的传感器在涵盖生理浓度的宽动态范围内表现出线性关系，从 1 ng ml 到 10 mg ml，灵敏度为 0.0914 mA（ng/ml）（cm）。基于 LDH 的传感器被发现对纤维蛋白原蛋白具有较低的检测限（LOD）为 0.097 ng ml 和较低的定量限（LOQ）为 0.294 ng ml（S/N = 3.3）。在存在各种干扰分析物如多巴胺、肾上腺素、血清素、葡萄糖、钾、氯和镁离子的情况下，传感器对纤维蛋白原的选择性更高。该传感器成功地用于模拟体液中纤维蛋白原的痕量检测，回收率在 99.5%至 102.5%之间，证明了二维 Ni-Fe 层状双氢氧化物和三维泡沫镍的协同组合作为电化学传感的有前途的平台具有巨大的临床应用潜力。
