Department of Integrated Traditional Chinese and Western Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China.
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Environ Pollut. 2024 Jan 1;340(Pt 2):122777. doi: 10.1016/j.envpol.2023.122777. Epub 2023 Oct 18.
The aim of this study was to conduct transcriptomic, proteomic, and metabolomic analysis to provide a comprehensive view of plant response to tetracycline stress. Pumpkin seeds were cultured for 7 days without or with tetracycline at 10 mg/L. Pumpkin roots showed excessive growth inhibition, but not yet strong growth restraining in cotyledons. Tetracycline affected the abundance of metabolites related to flavonoid biosynthesis and amino acid metabolism. Main changes of metabolites in flavonoid biosynthesis were consistent with mRNA changes. Amino acid changes are mainly mediated by proteins or mRNAs. To be specific, tetracycline treatment increased the levels of rutin, caffeate, cinnamaldehyde, 4-hydroxycinnamic acid, ferulic acid, naringenin, apigenin, luteolin, (-)-epigallocatechin, astragalin, L-serine, and glutathione and the transcript levels related to these compounds; and decreased the levels of indole pyruvate, indole acetaldehyde, L-arginine, S-adenosylhomocysteine, L-glutamine, and gamma-glutamylcysteine and the transcript levels related to these compounds. Tetracycline treatment also increased the levels of oxoglutaric acid, L-glutamic acid, gamma-aminobutyric acid, and gamma-glutamylalanine and enzymes involved in their production; and decreased the levels of L-isoleucine, L-valine, and L-leucine and enzymes involved in their production. We elucidated several biological processes (e.g. phenylpropanoid/flavonoid biosynthesis pathways, amino acid metabolic pathways) that were altered by tetracycline, and provided a multi-omic perspective on the mechanisms underlying the response to tetracycline stress in pumpkin roots. We provide a useful reference for the development of environmental quality management methods.
本研究旨在进行转录组学、蛋白质组学和代谢组学分析，提供植物对四环素胁迫反应的综合视图。南瓜种子在无四环素或 10mg/L 四环素的条件下培养 7 天。南瓜根表现出过度生长抑制，但子叶尚未出现强烈的生长抑制。四环素影响与类黄酮生物合成和氨基酸代谢相关的代谢物丰度。类黄酮生物合成中代谢物的主要变化与 mRNA 变化一致。氨基酸变化主要由蛋白质或 mRNAs 介导。具体而言，四环素处理增加了芦丁、咖啡酸、肉桂醛、4-羟基肉桂酸、阿魏酸、柚皮苷、芹菜素、木樨草素、(-)-表儿茶素、黄芪苷、L-丝氨酸和谷胱甘肽及其相关化合物的水平；并降低了吲哚丙酮酸、吲哚乙醛、L-精氨酸、S-腺苷同型半胱氨酸、L-谷氨酰胺和γ-谷氨酰半胱氨酸及其相关化合物的水平。四环素处理还增加了草酰谷氨酸、L-谷氨酸、γ-氨基丁酸和γ-谷氨酰丙氨酸及其相关酶的水平；并降低了 L-异亮氨酸、L-缬氨酸和 L-亮氨酸及其相关酶的水平。我们阐明了几个被四环素改变的生物学过程（例如苯丙烷类/类黄酮生物合成途径、氨基酸代谢途径），并从多组学角度提供了南瓜根对四环素胁迫反应的机制。我们为开发环境质量管理方法提供了有用的参考。
