Han Tao, Mi Zhaorong, Chen Zhe, Zhao Jinjin, Zhang Haiguang, Lv Yang, Du Shouyang, Bu Ruifang, Zhou Junguo, Li Xinzheng, Sun Zhiqiang, Chen Zhuo, Song Yiting, Zhang Jun, Hao Ruijuan, Ge Shidong
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Henan Institute of Science and Technology, Xinxiang 453003, China; Postdoctoral Research Station, Henan Agricultural University, Zhengzhou 450002, China.
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Henan Institute of Science and Technology, Xinxiang 453003, China.
J Hazard Mater. 2022 Aug 5;435:129019. doi: 10.1016/j.jhazmat.2022.129019. Epub 2022 Apr 29.
Environmental factors, including antibiotics such as tetracycline, can alter biological processes in plants. To ascertain how cell/tissue response to tetracycline, a multi-omic analysis was implemented to explore the molecular mechanism of tetracycline influencing the growth of ryegrass root. Tetracycline induced extensive changes in the root metabolome in plants, particularly impacting metabolites of flavonoid metabolic pathways, which were supported through consistent differences between transcriptome and proteome. Cross-comparison between mRNA and protein contents considered the authentication of congruence with related metabolites and revealed changes of several biological processes under tetracycline stress. Overall, we present an undemanding multi-omic strategy to survey the significant influence on the root under tetracycline stress.
包括四环素等抗生素在内的环境因素会改变植物中的生物过程。为了确定细胞/组织对四环素的反应，进行了多组学分析，以探究四环素影响黑麦草根系生长的分子机制。四环素诱导了植物根系代谢组的广泛变化，尤其影响黄酮类代谢途径的代谢物，转录组和蛋白质组之间的一致性差异证实了这一点。mRNA和蛋白质含量之间的交叉比较考虑了与相关代谢物一致性的验证，并揭示了四环素胁迫下几个生物过程的变化。总体而言，我们提出了一种简单的多组学策略，以研究四环素胁迫对根系的显著影响。
