新型节杆菌噬菌体 SWEP2 的分离、鉴定及基因组分析。
Isolation, characterization and genomic analysis of the novel Arthrobacter sp. phage SWEP2.
机构信息
College of Land Science and Technology, China Agricultural University, Beijing, 100193, China.
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
出版信息
Arch Virol. 2023 Oct 20;168(11):276. doi: 10.1007/s00705-023-05898-0.
A new virulent phage, SWEP2, infecting the Arthrobacter sp. 5B strain, was isolated from black soil samples in northeastern China. SWEP2 has a latent period of 80 min and a burst size of 45 PFU (evaluated at an MOI of 0.1). Genomic analysis revealed that the 43,398-bp dsDNA genome of phage SWEP2 contains 64 open reading frames (ORFs) and one tRNA gene. Phylogenetic analysis indicated a close relationship between SWEP2 and Arthrobacter phage Liebe, with 82.98% identity and a query coverage of 48%. Based on its distinct phenotypic and genetic characteristics, SWEP2 is identified as a novel Arthrobacter phage.
一种新的烈性噬菌体 SWEP2 从中国东北的黑土样本中分离出来，它感染节杆菌属 5B 菌株。SWEP2 的潜伏期为 80 分钟，爆发量为 45PFU（在 MOI 为 0.1 的情况下评估）。基因组分析表明，噬菌体 SWEP2 的 43398bpdsDNA 基因组包含 64 个开放阅读框（ORFs）和一个 tRNA 基因。系统发育分析表明，SWEP2 与节杆菌噬菌体 Liebe 密切相关，同源性为 82.98%，查询覆盖率为 48%。基于其独特的表型和遗传特征，SWEP2 被鉴定为一种新型节杆菌噬菌体。
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