Division of Agricultural Biotechnology, School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda Educational and Research Institute, Ramakrishna Mission Ashrama, Narendrapur, Kolkata, India.
Division of Rural Development, School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda Educational and Research Institute, Ramakrishna Mission Ashrama, Narendrapur, Kolkata, India.
World J Microbiol Biotechnol. 2023 Oct 21;39(12):351. doi: 10.1007/s11274-023-03809-8.
The hardening step of micropropagation is crucial to make the in vitro raised plants mature and further enhancing their survivability in the external environment. Auxin regulates various root physiological parameters in plant systems. Therefore, the present study aimed to assess the impact of three vermicompost-derived IAA-releasing microbial strains, designated S1, S2, and S3, as biofertilizers on in vitro raised banana plantlets during primary hardening. The High-Performance Thin-Layer Chromatography (HPTLC) analysis of these strains revealed a higher IAA content for S1 and S2 than that of S3 after 144 h of incubation. In total, seven different treatments were applied to banana plantlets, and significant variations were observed in all plant growth parameters for all treatments except autoclaved cocopeat (100%) mixed with autoclaved vermicompost (100%) at a 1:1 ratio. Among these treatments, the application of S3 biofertilizer: autoclaved cocopeat (1:1), followed by S2 biofertlizer: autoclaved cocopeat (1:1), was found to be better than other treatments for root numbers per plant, root length per plant, root volume, and chlorophyll content. These findings have confirmed the beneficial effects of microbial strains on plant systems and propose a link between root improvement and bacterial auxin. Further, these strains were identified at the molecular level as Bacillus sp. As per our knowledge, this is the first report of Bacillus strains isolated from vermicompost and applied as biofertilizer along with cocopeat for the primary hardening of banana. This unique approach may be adopted to improve the quality of plants during hardening, which increases their survival under abiotic stresses.
微繁殖的硬化步骤对于使体外培养的植物成熟并进一步提高其在外部环境中的生存能力至关重要。生长素调节植物系统中的各种根生理参数。因此，本研究旨在评估三种来源于蚯蚓堆肥的 IAA 释放微生物菌株（S1、S2 和 S3）作为生物肥料对初级硬化过程中体外培养的香蕉苗的影响。这些菌株的高效薄层层析（HPTLC）分析显示，在 144 小时的孵育后，S1 和 S2 的 IAA 含量高于 S3。总共对香蕉苗进行了七种不同的处理，除了 100%的高压灭菌椰糠与 100%的高压灭菌蚯蚓堆肥以 1:1 的比例混合外，所有处理的所有植物生长参数都有显著差异。在这些处理中，S3 生物肥料：高压灭菌椰糠（1:1），其次是 S2 生物肥料：高压灭菌椰糠（1:1），被发现比其他处理更有利于每株植物的根数、每株植物的根长、根体积和叶绿素含量。这些发现证实了微生物菌株对植物系统的有益影响，并提出了根改良与细菌生长素之间的联系。此外，这些菌株在分子水平上被鉴定为芽孢杆菌属。据我们所知，这是首次从蚯蚓堆肥中分离出芽孢杆菌属菌株并与椰糠一起作为生物肥料应用于香蕉的初级硬化。这种独特的方法可以用来提高硬化过程中植物的质量，从而提高它们在非生物胁迫下的存活率。
