Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Department of Neurosurgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China.
Funct Integr Genomics. 2023 Oct 21;23(4):322. doi: 10.1007/s10142-023-01246-2.
Brain glioma is a common gynecological tumor. MicroRNA (miRNA) plays a very important role in the pathogenesis and development of tumors. It was found that glycolysis played important regulatory roles in tumor growth. The present study aims to investigate the expression pattern of miR-21-5p in brain glioma cells. We examined miR-21-5p and PFKFB2 levels in brain glioma cells via qRT-PCR. Then we performed CCK-8 and Transwell migration assays and determined glucose uptake and lactose production to unveil the properties of miR-21-5p in invasion, cell viability, along with glycolysis in brain glioma cells. Luciferase activity assay was implemented to elucidate if PFKFB2 was a miR-21-5p target gene. Western blotting and qRT-PCR were executed to further validate that miR-21-5p targeted PFKFB2. We repeated these functional assays to observe whether miR-21-5p could impede the function of PFKFB2. qRT-PCR signified that miR-21-5p was elevated in brain glioma tissues in contrast to matching adjacent normal tissues. Functional assays disclosed that elevation of miR-21-5p promoted cell viability, invasion, together with glycolysis. Luciferase assay indicated that PFKFB2 was a miR-21-5p target gene. Moreover, miR-21-inhibit could hinder cell viability, invasion, and glycolysis triggered by overexpression of PFKFB2 in brain glioma cells. miR-21-5p level is elevated in brain glioma and can impede brain glioma cell growth via regulating the glycolysis mediated by PFKFB2, thus is a potential target of treating brain glioma.
脑胶质瘤是一种常见的妇科肿瘤。微小 RNA（miRNA）在肿瘤的发病机制和发展中起着非常重要的作用。人们发现糖酵解在肿瘤生长中起着重要的调节作用。本研究旨在探讨 miR-21-5p 在脑胶质瘤细胞中的表达模式。我们通过 qRT-PCR 检测了脑胶质瘤细胞中 miR-21-5p 和 PFKFB2 的水平。然后，我们进行了 CCK-8 和 Transwell 迁移实验，并测定了葡萄糖摄取和乳糖生成，以揭示 miR-21-5p 在脑胶质瘤细胞侵袭、细胞活力和糖酵解中的特性。通过荧光素酶活性测定阐明 PFKFB2 是否是 miR-21-5p 的靶基因。Western blot 和 qRT-PCR 进一步验证了 miR-21-5p 靶向 PFKFB2。我们重复了这些功能实验，观察 miR-21-5p 是否可以抑制 PFKFB2 的功能。qRT-PCR 表明，与匹配的相邻正常组织相比，miR-21-5p 在脑胶质瘤组织中升高。功能实验表明，miR-21-5p 的升高促进了细胞活力、侵袭和糖酵解。荧光素酶测定表明，PFKFB2 是 miR-21-5p 的靶基因。此外，miR-21 抑制可以阻止脑胶质瘤细胞中 PFKFB2 过表达引起的细胞活力、侵袭和糖酵解。miR-21-5p 在脑胶质瘤中的水平升高，通过调节 PFKFB2 介导的糖酵解，可以抑制脑胶质瘤细胞的生长，因此是治疗脑胶质瘤的潜在靶点。
