利用电子显微镜分析小鼠大脑中的自噬体和线粒体自噬体。
Analyzing autophagosomes and mitophagosomes in the mouse brain using electron microscopy.
机构信息
Section on Synapse Development Plasticity, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA.
Advanced Imaging Core, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
STAR Protoc. 2022 Feb 3;3(1):101154. doi: 10.1016/j.xpro.2022.101154. eCollection 2022 Mar 18.
Electron microscopy (EM) is considered the gold standard for studying macroautophagy and mitophagy, essential cellular processes for brain health. Here, we present a protocol using EM to analyze autophagosomes and mitophagosomes in the mouse amygdala. We describe the preparation of brain sections, followed by staining and EM imaging. We then detail the steps to identify and analyze autophagosome-like and mitophagosome-like structures. This protocol can be easily adapted to analyze autophagosomes and mitophagosomes in other mouse brain regions. For complete details on the use and execution of this protocol, please refer to Duan et al. (2021).
电子显微镜（EM）被认为是研究巨自噬和线粒体自噬的金标准，这是大脑健康所必需的细胞过程。在这里，我们介绍了一种使用 EM 分析小鼠杏仁核中自噬体和线粒体自噬体的方案。我们描述了脑切片的制备，然后进行染色和 EM 成像。然后，我们详细介绍了识别和分析类自噬体和类线粒体自噬体结构的步骤。该方案可以很容易地适应于分析其他小鼠脑区的自噬体和线粒体自噬体。有关该方案使用和执行的完整详细信息，请参阅 Duan 等人（2021 年）。
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