Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Department of Immunology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
STAR Protoc. 2023 Dec 15;4(4):102648. doi: 10.1016/j.xpro.2023.102648. Epub 2023 Oct 21.
Environmental nano- and microplastics (NMPs) pose serious environmental issues, yet there is no established technique to assess their impact on health through oral ingestion. Here, we present a protocol to assess the impact of NMPs in the intestinal immune microenvironments by employing chronic exposure to NMPs in a mouse model. We describe steps for administration of NMPs, feces and tissue collection, and colonic gut digestion. We then detail procedures for isolation of intestinal immune cells and RNA isolation. For complete details on the use and execution of this protocol, please refer to Harusato et al..
环境纳米和微塑料（NMPs）带来了严重的环境问题，但目前还没有建立评估其通过口服摄入对健康影响的技术。在这里，我们通过在小鼠模型中进行慢性 NMP 暴露，提出了一种评估 NMP 对肠道免疫微环境影响的方案。我们描述了给予 NMP、收集粪便和组织以及结肠肠道消化的步骤。然后，我们详细介绍了分离肠道免疫细胞和提取 RNA 的程序。如需了解本方案的使用和执行的详细信息，请参考 Harusato 等人的研究。
