Wang Yunzheng, Hou Chen, Dai Yuqi, Chu Lanling, Geng Shiwei, Zheng Shenglan, Kang Xuejun
School of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.
School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Anal Methods. 2023 Jan 26;15(4):472-481. doi: 10.1039/d2ay01753a.
A novel analytical proposal based on nanofiber-packed solid-phase extraction coupled with high performance liquid chromatography-fluorescence detector (HPLC-FLD) has been successfully developed for determining aflatoxin B1 (AFB1) in foods. Four types of nanofibers, including polystyrene (PS) nanofibers, polypyrrole (PPY) nanofibers, polystyrene-acrylic resin (PS-AR) nanofibers, and polystyrene-polyvinyl pyrrolidone (PS-PVP) nanofibers, were fabricated by electrospinning and utilized to prepare a home-made extraction device. In this study, the factors of different fibers, namely, fiber dosage, pH of extraction solution, type of salt ion, concentration of salt ion, and volume of the eluent were optimized. Under optimized conditions, the method showed good linearity in the range of 0.1-40 ng mL with a correlation coefficient greater than 0.999 and good inter-day accuracy (90.8-112.7% recovery) and precision (1.8-3.6% intra-day RSDs, 2.6% inter-day RSD), and the limit of detection (LOD) was 0.05 ng mL. Due to its cost-effective, time-saving, environmentally friendly, and simple performance, it has the potential to be utilized to determine aflatoxins in complicated matrices.
一种基于纳米纤维填充固相萃取结合高效液相色谱 - 荧光检测器（HPLC - FLD）的新型分析方法已成功开发用于测定食品中的黄曲霉毒素B1（AFB1）。通过静电纺丝制备了四种类型的纳米纤维，包括聚苯乙烯（PS）纳米纤维、聚吡咯（PPY）纳米纤维、聚苯乙烯 - 丙烯酸树脂（PS - AR）纳米纤维和聚苯乙烯 - 聚乙烯吡咯烷酮（PS - PVP）纳米纤维，并用于制备自制萃取装置。在本研究中，对不同纤维的因素，即纤维用量、萃取溶液的pH值、盐离子类型、盐离子浓度和洗脱剂体积进行了优化。在优化条件下，该方法在0.1 - 40 ng/mL范围内具有良好的线性，相关系数大于0.999，日间准确度良好（回收率为90.8 - 112.7%）和精密度良好（日内相对标准偏差为1.8 - 3.6%，日间相对标准偏差为2.6%），检测限（LOD）为0.05 ng/mL。由于其具有成本效益、省时、环保且操作简单的特点，它有潜力用于测定复杂基质中的黄曲霉毒素。
