Magee-Womens Research Institute, Pittsburgh, PA, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA; Joint CMU-Pitt PhD Program in Computational Biology, Pittsburgh, PA, USA.
Placenta. 2023 Nov;143:87-90. doi: 10.1016/j.placenta.2023.10.010. Epub 2023 Oct 17.
Trophoblast injury is central to clinically relevant placenta dysfunction. We hypothesized that the mRNA of primary human trophoblasts, exposed to distinct injuries in vitro, capture transcriptome patterns of placental biopsies obtained from common obstetrical syndromes. We deployed a CIBERSORTx deconvolution method to correlate trophoblastic RNAseq-based expression matrices with the transcriptome of omics-defined placental dysfunction patterns in vivo. We found distinct trophoblast injury patterns in placental biopsies from women with fetal growth restriction and a hypertensive disorder, or in biopsies clustered by their omics analysis. Our RNAseq data are useful for defining the contribution of trophoblast injuries to placental dysfunction syndromes.
滋养层细胞损伤是与临床相关的胎盘功能障碍的核心。我们假设，在体外受到不同损伤的原代人滋养层细胞，能够捕获从常见产科综合征获得的胎盘活检的转录组模式。我们使用 CIBERSORTx 去卷积方法，将基于滋养层 RNAseq 的表达矩阵与体内基于组学定义的胎盘功能障碍模式的转录组相关联。我们在胎儿生长受限和高血压疾病妇女的胎盘活检中发现了不同的滋养层损伤模式，或在通过组学分析聚类的活检中发现了不同的滋养层损伤模式。我们的 RNAseq 数据可用于定义滋养层损伤对胎盘功能障碍综合征的贡献。
