Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, People's Republic of China.
Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, People's Republic of China.
Fitoterapia. 2023 Dec;171:105710. doi: 10.1016/j.fitote.2023.105710. Epub 2023 Oct 20.
Six new sesquiterpenes, fusarchlamols A-F (1, 2, 4-7); one new natural product of sesquiterpenoid, methyltricinonoate (3); and ten known compounds were found from Fusarium sp. cultured in two different media by the one strain many compounds strategy. The compounds (1, 2, and 4-11) were isolated from Fusarium sp. in PDB medium, and compounds (3-5, 8, and 10-17) were discovered from Fusarium sp. in coffee medium. Additionally, the configuration of 8 was first reported in the research by Mosher's method. The structures were established by 1D, 2D NMR, mass spectrometry, calculated ECD spectra, and Mosher's method. Compounds 1, 2, 6/7, 12, and 16 indicated significant antifungal activities against the phytopathogen Alternaria alternata isolated from Coffea arabica with MICs of 1 μg/mL. The investigation on the anti-phytopathogen activity of metabolites can provide lead compounds for agrochemicals.
从通过“一菌多产物”策略在两种不同培养基中培养的尖孢镰刀菌中发现了 6 种新的倍半萜类化合物（fusarchlamols A-F，1、2、4-7）；一种新的倍半萜天然产物，甲基tricinoonate（3）；以及 10 种已知化合物。化合物（1、2 和 4-11）从 PDB 培养基中的尖孢镰刀菌中分离得到，而化合物（3-5、8 和 10-17）则从咖啡培养基中的尖孢镰刀菌中发现。此外，8 的构型是首次通过 Mosher 法在研究中报道的。通过 1D、2D NMR、质谱、计算 ECD 光谱和 Mosher 法确定了结构。化合物 1、2、6/7、12 和 16 对从阿拉比卡咖啡中分离出的植物病原菌链格孢属显示出显著的抗真菌活性，MIC 值为 1μg/mL。代谢产物抗植物病原菌活性的研究可为农用化学品提供先导化合物。
