School of Chemical Science and Technology, Yunnan University, Kunming 650500, People's Republic of China.
College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, People's Republic of China.
J Agric Food Chem. 2024 Oct 2;72(39):21585-21593. doi: 10.1021/acs.jafc.4c04237. Epub 2024 Sep 18.
Two endophytes from the same host were isolated and cultured separately. Three new eremophilane sesquiterpenoids (), three new furan derivates (, -), one new polyketide (), and four known compounds (, , , ) from sp. and two new 10-membered macrolides (-), a new liner polyketide (), a new benzofuran (), and six known compounds (-) from were isolated. The structures of the isolated compounds were determined by spectroscopic methods, NMR calculations, and ECD calculations. The compounds -, -, , and - showed significant antiphytopathogenic effects against mycotoxigenic sp. comparable to the activity of nystatin (positive control). Compounds , , , , and indicated inhibitions against phytopathogen with MICs < 10 μg/mL. In addition, the compounds with weak antifungal activities from two endophytes were mixed to test their antifungal activity. The results showed that the metabolites from two endophytes had synergistic antifungal effects, and the beneficial interactions between natural products can induce more antifungal effects against plant pathogens than that of single compounds.
从同一宿主中分离并培养了两种内生菌。从 中分离得到了三种新的半日花烷倍半萜（）、三种新的呋喃衍生物（、-）、一种新的聚酮化合物（）和四种已知化合物（、、、），从 中分离得到了两种新的 10 元大环内酯（-）、一种新的线性聚酮化合物（）、一种新的苯并呋喃（）和六种已知化合物（-）。通过光谱方法、NMR 计算和 ECD 计算确定了分离化合物的结构。化合物-、-、-、-对产毒真菌 表现出与制霉菌素（阳性对照）相当的抗真菌效果。化合物-、-、-、-对植物病原菌 表现出抑制作用，MIC 值均小于 10μg/mL。此外，将两种内生菌中具有较弱抗真菌活性的化合物混合测试其抗真菌活性。结果表明，两种内生菌的代谢产物具有协同抗真菌作用，天然产物之间的有益相互作用可诱导出比单一化合物更强的抗植物病原菌作用。
