The Laboratory of Effective Substances of Jiangxi Genuine Medicinal Materials, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China.
The Laboratory of Effective Substances of Jiangxi Genuine Medicinal Materials, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
Fitoterapia. 2023 Dec;171:105711. doi: 10.1016/j.fitote.2023.105711. Epub 2023 Oct 20.
Motivated by the potential anti-inflammatory effect of the crude extract of endophytic fungus Microdiplodia sp. CJ01 derived from Camellia sinensis, chemical investigation of the extract of Microdiplodia sp. CJ01 led to the isolation and identification of sixteen terpenoids, including five undescribed eremophilane sesquiterpenoids named microdiplodins A-E (1-5), one undescribed meroterpenoid 13-carboxymacrophorin A (13), seven known eremophilane sesquiterpenoids (6-12), and three known meroterpenoids (14-16). The structures of these compounds were elucidated based on extensive spectroscopic analysis, including nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) data. Their absolute configurations were determined by calculational and experimental electronic circular dichroism (ECD) data. Anti-inflammatory activity assays revealed that compounds 3, 4, 14-16 exhibited moderate inhibitory effects on the production of nitric oxide (NO) in the lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells.
受来源于茶树内生真菌 Microdiplodia sp. CJ01 的粗提物具有抗炎作用的启发，对 Microdiplodia sp. CJ01 的提取物进行了化学研究，分离并鉴定了 16 种萜类化合物，包括 5 种未命名的埃罗啡烷倍半萜类化合物（1-5）、1 种未命名的 13-羧基大孢菌素 A 型倍半萜-二萜（13）、7 种已知的埃罗啡烷倍半萜类化合物（6-12）和 3 种已知的倍半萜类化合物（14-16）。这些化合物的结构是基于广泛的光谱分析，包括核磁共振（NMR）和高分辨率质谱（HRMS）数据来阐明的。它们的绝对构型是通过计算和实验电子圆二色性（ECD）数据确定的。抗炎活性测定表明，化合物 3、4、14-16 对脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）的产生具有中等抑制作用。
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