Department of Botany, University of British Columbia, Vancouver, V6T 1Z4, Canada.
Laboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University, Linyi, 276005, China.
J Integr Plant Biol. 2023 Dec;65(12):2660-2671. doi: 10.1111/jipb.13577. Epub 2023 Nov 27.
N-Hydroxypipecolic acid (NHP) is a signaling molecule crucial for systemic acquired resistance (SAR), a systemic immune response in plants that provides long-lasting and broad-spectrum protection against secondary pathogen infections. To identify negative regulators of NHP biosynthesis, we performed a forward genetic screen to search for mutants with elevated expression of the NHP biosynthesis gene FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1). Analysis of two constitutive expression of FMO1 (cef) and one induced expression of FMO1 (ief) mutants revealed that the AIPP3-PHD2-CPL2 protein complex, which is involved in the recognition of the histone modification H3K27me3 and transcriptional repression, contributes to the negative regulation of FMO1 expression and NHP biosynthesis. Our study suggests that epigenetic regulation plays a crucial role in controlling FMO1 expression and NHP levels in plants.
N-羟基哌啶酸（NHP）是一种信号分子，对系统性获得抗性（SAR）至关重要，SAR 是植物的一种系统性免疫反应，可提供针对二次病原体感染的持久和广谱保护。为了鉴定 NHP 生物合成的负调控因子，我们进行了正向遗传筛选，以寻找 NHP 生物合成基因黄素依赖性单加氧酶 1（FMO1）表达升高的突变体。对两个组成型表达 FMO1（cef）和一个诱导型表达 FMO1（ief）突变体的分析表明，参与识别组蛋白修饰 H3K27me3 和转录抑制的 AIPP3-PHD2-CPL2 蛋白复合物有助于 FMO1 表达和 NHP 生物合成的负调控。我们的研究表明，表观遗传调控在植物中控制 FMO1 表达和 NHP 水平方面起着至关重要的作用。
