Herrera-Martínez Aura D, Castillo-Peinado Laura L S, Molina-Puerta María J, Calañas-Continente Alfonso, Membrives Antonio, Castilla Juan, Camacho Cardenosa Marta, Casado-Díaz Antonio, Gálvez-Moreno María A, Gahete Manuel D, Quesada Gómez José Manuel, Bouillon Roger, Priego-Capote Feliciano, Luque Raúl M
Maimonides Institute for Biomedical Research of Cordoba (IMIBIC), Córdoba, Spain.
Endocrinology and Nutrition Service, Reina Sofia University Hospital, Córdoba, Spain.
Front Endocrinol (Lausanne). 2023 Oct 6;14:1243906. doi: 10.3389/fendo.2023.1243906. eCollection 2023.
Obesity (OB) is a chronic metabolic disease with important associated comorbidities and mortality. Vitamin D supplementation is frequently administered after bariatric surgery (BS), so as to reduce OB-related complications, maybe including chronic inflammation.
This study aimed to explore relations between vitamin D metabolites and components of the inflammasome machinery in OB before and after BS and their relations with the improvement of metabolic comorbidities.
Epidemiological/clinical/anthropometric/biochemical evaluation was performed in patients with OB at baseline and 6 months after BS. Evaluation of i) vitamin-D metabolites in plasma and ii) components of the inflammasome machinery and inflammatory-associated factors [NOD-like-receptors (NLRs), inflammasome-activation-components, cytokines and inflammation/apoptosis-related components, and cell-cycle and DNA-damage regulators] in peripheral blood mononuclear cells (PBMCs) was performed at baseline and 6 months after BS. Clinical and molecular correlations/associations were analyzed.
Significant correlations between vitamin D metabolites and inflammasome-machinery components were observed at baseline, and these correlations were significantly reduced 6 months after BS in parallel to a decrease in inflammation markers, fat mass, and body weight. Treatment with calcifediol remarkably increased 25OHD levels, despite 24,25(OH)D remained stable after BS. Several inflammasome-machinery components were associated with improvement in metabolic comorbidities, especially hypertension and dyslipidemia.
The beneficial effects of vitamin D on OB-related comorbidities after BS patients are associated with significant changes in the molecular expression of key inflammasome-machinery components. The expression profile of these inflammasome components can be dynamically modulated in PBMCs after BS and vitamin D supplementation, suggesting that this profile could likely serve as a sensor and early predictor of the reversal of OB-related complications after BS.
肥胖症（OB）是一种慢性代谢性疾病，伴有重要的相关合并症和死亡率。减肥手术后（BS）经常补充维生素D，以减少与肥胖症相关的并发症，可能包括慢性炎症。
本研究旨在探讨减肥手术前后肥胖症患者体内维生素D代谢产物与炎性小体机制成分之间的关系，以及它们与代谢合并症改善情况的关系。
对肥胖症患者在基线时和减肥手术后6个月进行流行病学/临床/人体测量学/生化评估。在基线时和减肥手术后6个月，对以下指标进行评估：i）血浆中的维生素D代谢产物；ii）外周血单核细胞（PBMCs）中的炎性小体机制成分和炎症相关因子[核苷酸结合寡聚化结构域样受体（NLRs）、炎性小体激活成分、细胞因子以及炎症/凋亡相关成分，还有细胞周期和DNA损伤调节因子]。分析临床和分子方面的相关性/关联性。
在基线时观察到维生素D代谢产物与炎性小体机制成分之间存在显著相关性，减肥手术后6个月，随着炎症标志物、脂肪量和体重的下降，这些相关性显著降低。尽管减肥手术后24,25(OH)D保持稳定，但骨化二醇治疗显著提高了25OHD水平。几种炎性小体机制成分与代谢合并症的改善有关，尤其是高血压和血脂异常。
减肥手术后患者体内，维生素D对肥胖症相关合并症的有益作用与关键炎性小体机制成分的分子表达显著变化有关。减肥手术和补充维生素D后，外周血单核细胞中这些炎性小体成分的表达谱可动态调节，这表明该表达谱可能是减肥手术后肥胖症相关并发症逆转的一个监测指标和早期预测指标。
