nanoextract as a piezoelectric ultrasonic scaler coolant in gingivitis treatment in a Wistar rat model.

OBJECTIVES

This research was aimed at investigating the effects of various concentrations of nanoextract as a piezoelectric scaler coolant on gingivitis healing in a Wistar rat model.

METHODS

A piezoelectric ultrasonic scaler coolant was made from nanoextract through ionic gelation. Experiments were conducted in 45 adult male Wistar rats divided into three groups treated with nanoextract coolant (25%, 30%, and 35% concentrations), and negative and positive control groups. A silk ligature was used to trap debris and induce gingivitis in the maxillary incisors of the rats. Scaling was conducted with a piezoelectric ultrasonic scaler after the respective treatment for each group. Data were collected on days 3, 5, 7, 14, and 21 after treatment. Observations were collected with an Optilab® camera at 400× magnification. Angiogenesis and neutrophil data were analyzed with two-way analysis of variance (ANOVA) and post hoc Duncan tests at a 95% significance level.

RESULTS

Use of nanoextract as a piezoelectric ultrasonic coolant accelerated gingivitis healing in Wistar rats, particularly at a 25% concentration. Two-way ANOVA indicated a significant difference in angiogenesis and neutrophil counts between the control group and each treatment group (p < 0.05). Duncan's post-hoc test showed significant differences in mean neutrophil numbers and angiogenesis among groups on days 3, 5, 7, 14, and 21. The group treated with 25% nanoextract concentration showed no significant differences with respect to the positive control group.

CONCLUSIONS

Use of nanoextract as a piezoelectric ultrasonic coolant had good therapeutic results in promoting gingivitis healing. nanoextract may potentially resolve inflammation in gingivitis by modulating neutrophils and angiogenesis.