Wan Lidi, Searleman Adam C, Ma Yajun, Wong Jonathan H, Williams Judith, Murphy Mark E, Du Jiang, Chang Eric Y, Tang Guangyu
Department of Radiology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.
Department of Radiology, University of California, San Diego, CA, USA.
Quant Imaging Med Surg. 2023 Oct 1;13(10):6942-6951. doi: 10.21037/qims-23-359. Epub 2023 Sep 8.
The effect of dehydration of cartilage samples and rehydration with native synovial fluid or normal saline on quantitative ultrashort echo time (UTE) biomarkers are unknown. We aimed to investigate the effect of cartilage dehydration-rehydration on UTE biomarkers and to compare the rehydration capabilities of native synovial fluid and normal saline.
A total of 37 cartilage samples were harvested from patients (n=5) who underwent total knee replacement. Fresh cartilage samples were exposed to air to dehydrate for 2 hours after baseline magnetic resonance (MR) scanning, then randomly divided into two groups: one soaking in native synovial fluid (n=17) and the other in normal saline (n=20) to rehydrate for 4 hours. UTE-based biomarkers [T, adiabatic T (AdiabT), macromolecular fraction (MMF), magnetization transfer ratio (MTR), and T*] and sample weights were evaluated for fresh, dehydrated, and rehydrated cartilage samples. Differences and agreements between groups were assessed using the values of fresh cartilage samples as reference standard.
Dehydrating in air for 2 hours resulted in significant weight loss (P=0.000). T, AdiabT, and T* decreased significantly while MMF and MTR increased significantly (all P<0.02). Non-significant differences were observed in cartilage weights after rehydrating in both synovial fluid and normal saline, with P values being 0.204 and 0.769, respectively. There were no significant differences in T, AdiabT, MMF, and MTR after rehydrating in synovial fluid (P>0.0167, with Bonferroni correction) while T* (P=0.001) still had significant differences compared with fresh samples. However, no significant differences were detected for any of the evaluated UTE biomarkers after rehydrating in normal saline (all P>0.05). No differences were detected in the agreement of UTE biomarker measurements between fresh samples and samples rehydrated with synovial fluid and normal saline.
Cartilage dehydration resulted in significant changes in UTE biomarkers. Rehydrating with synovial fluid or normal saline had non-significant effect on all the evaluated UTE biomarkers except T* values, which still had significant differences compared with fresh samples after rehydrating with synovial fluid. No significant difference was observed in the rehydration capabilities of native synovial fluid and normal saline.
软骨样本脱水以及用天然滑液或生理盐水复水对定量超短回波时间（UTE）生物标志物的影响尚不清楚。我们旨在研究软骨脱水 - 复水对UTE生物标志物的影响，并比较天然滑液和生理盐水的复水能力。
从接受全膝关节置换术的患者（n = 5）中总共获取37个软骨样本。在基线磁共振（MR）扫描后，将新鲜软骨样本暴露于空气中脱水2小时，然后随机分为两组：一组浸泡在天然滑液中（n = 17），另一组浸泡在生理盐水中（n = 20）复水4小时。对新鲜、脱水和复水后的软骨样本评估基于UTE的生物标志物[T、绝热T（AdiabT）、大分子分数（MMF）、磁化传递比（MTR）和T*]以及样本重量。以新鲜软骨样本的值作为参考标准评估组间差异和一致性。
在空气中脱水2小时导致显著的重量减轻（P = 0.000）。T、AdiabT和T显著降低，而MMF和MTR显著增加（所有P < 0.02）。用滑液和生理盐水复水后，软骨重量无显著差异，P值分别为0.204和0.769。用滑液复水后，T、AdiabT、MMF和MTR无显著差异（P > 0.0167，采用Bonferroni校正），而T（P = 0.001）与新鲜样本相比仍有显著差异。然而，用生理盐水复水后，所评估的任何UTE生物标志物均未检测到显著差异（所有P > 0.05）。新鲜样本与用滑液和生理盐水复水后的样本之间，UTE生物标志物测量的一致性未检测到差异。
软骨脱水导致UTE生物标志物发生显著变化。用滑液或生理盐水复水对所有评估的UTE生物标志物（除T值外）无显著影响，T值在用滑液复水后与新鲜样本相比仍有显著差异。天然滑液和生理盐水的复水能力未观察到显著差异。
