绵羊出生后骨骼肌生长过程中的全基因组表观遗传学动态。
Genome-wide epigenetic dynamics during postnatal skeletal muscle growth in Hu sheep.
机构信息
Beijing Key Laboratory of Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing, China.
National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
出版信息
Commun Biol. 2023 Oct 23;6(1):1077. doi: 10.1038/s42003-023-05439-0.
Hypertrophy and fiber transformation are two prominent features of postnatal skeletal muscle development. However, the role of epigenetic modifications is less understood. ATAC-seq, whole genome bisulfite sequencing, and RNA-seq were applied to investigate the epigenetic dynamics of muscle in Hu sheep at 3 days, 3 months, 6 months, and 12 months after birth. All 6865 differentially expressed genes were assigned into three distinct tendencies, highlighting the balanced protein synthesis, accumulated immune activities, and restrained cell division in postnatal development. We identified 3742 differentially accessible regions and 11799 differentially methylated regions that were associated with muscle-development-related pathways in certain stages, like D3-M6. Transcription factor network analysis, based on genomic loci with high chromatin accessibility and low methylation, showed that ARID5B, MYOG, and ENO1 were associated with muscle hypertrophy, while NR1D1, FADS1, ZFP36L2, and SLC25A1 were associated with muscle fiber transformation. Taken together, these results suggest that DNA methylation and chromatin accessibility contributed toward regulating the growth and fiber transformation of postnatal skeletal muscle in Hu sheep.
肥大和纤维转化是出生后骨骼肌发育的两个突出特征。然而，表观遗传修饰的作用还不太清楚。本研究应用 ATAC-seq、全基因组亚硫酸氢盐测序和 RNA-seq 来研究湖羊出生后 3 天、3 个月、6 个月和 12 个月时肌肉的表观遗传动态。所有 6865 个差异表达基因被分为三种不同的趋势，突出了出生后发育过程中蛋白质合成的平衡、免疫活性的积累和细胞分裂的抑制。我们鉴定了 3742 个差异可及区域和 11799 个差异甲基化区域，这些区域与特定阶段（如 D3-M6）的肌肉发育相关途径有关。基于具有高染色质可及性和低甲基化的基因组位点的转录因子网络分析表明，ARID5B、MYOG 和 ENO1 与肌肉肥大有关，而 NR1D1、FADS1、ZFP36L2 和 SLC25A1 与肌肉纤维转化有关。总之，这些结果表明，DNA 甲基化和染色质可及性有助于调节湖羊出生后骨骼肌的生长和纤维转化。
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