Herschman H R, Brankow D W
Science. 1986 Dec 12;234(4782):1385-8. doi: 10.1126/science.3787250.
Transformation of C3H10T1/2 cells by exposure to ultraviolet (UV) irradiation followed by tetradecanoyl phorbol acetate (TPA) has been used as a model of two-stage carcinogenesis. However, cells cloned from UV-TPA-induced foci (UV-TDTx cells) had a unique phenotype. Cloned UV-TDTx cells appeared transformed in pure culture but were unable to form foci when cocultured with C3H10T1/2 cells. However, in the presence of TPA, UV-TDTx cells form foci in mixed culture with C3H10T1/2 cells. This phenotype was the only one observed for UV-TPA transformants. These data suggest that communal suppression of cell division is a discrete phenomenon that must be overcome as one step in the multistage process of transformation, and this protocol permits the routine isolation of transformed cells responsive to density-dependent growth suppression.
通过紫外线（UV）照射后再用十四酰佛波醇乙酸酯（TPA）处理来转化C3H10T1/2细胞，已被用作两阶段致癌作用的模型。然而，从UV-TPA诱导的灶克隆得到的细胞（UV-TDTx细胞）具有独特的表型。克隆的UV-TDTx细胞在纯培养中呈现转化状态，但与C3H10T1/2细胞共培养时无法形成灶。然而，在TPA存在的情况下，UV-TDTx细胞与C3H10T1/2细胞混合培养时能形成灶。这种表型是UV-TPA转化体中观察到的唯一一种。这些数据表明，细胞分裂的群体抑制是一种离散现象，在多阶段转化过程中作为一个步骤必须被克服，并且该方案允许常规分离对密度依赖性生长抑制有反应的转化细胞。
