Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Vet Med Sci. 2023 Nov;9(6):2594-2599. doi: 10.1002/vms3.1305. Epub 2023 Oct 24.
Fungal and yeast infections, including those caused by Malassezia spp., are becoming increasingly difficult to treat, likely due to the occurrence of drug resistance.
This study aimed to evaluate the antifungal effects of omeprazole (OME), a proton pump inhibitor, against antifungal-resistant Malassezia pachydermatis and to investigate the potential synergistic effects between OME and other antifungal compounds.
In total, 15 samples of M. pachydermatis isolated from the skin of dogs were tested. The susceptibility of M. pachydermatis to itraconazole, ketoconazole, miconazole, terbinafine and OME was assessed using a modified broth microdilution (BM) method. The in vitro efficacy of OME alone and in combination with other antifungal compounds was evaluated for all isolates using the BM chequerboard method. The data obtained were analysed using the fractional inhibitory concentration index (FICI).
The minimum inhibitory concentration (MIC) values of antifungal compounds and OME against quality control strain (M. pachydermatis CBS1879) were lower than the MIC values of same drugs against clinically collected strains. There was no significant difference in MIC values between drugs alone and combination. According to the analysis by the FICI method, no interaction was observed with OME and antifungal compounds.
Most M. pachydermatis strains were resistant to azole antifungal compounds. OME exerted antifungal effects against Malassezia spp. and even showed good effects on antifungal-resistant strains. No synergistic effects were observed between the antifungal compounds and OME.
真菌和酵母感染，包括马拉色菌引起的感染，越来越难以治疗，这可能是由于耐药性的发生。
本研究旨在评估质子泵抑制剂奥美拉唑（OME）对抗真菌耐药马拉色菌的抗真菌作用，并研究 OME 与其他抗真菌化合物之间的潜在协同作用。
共检测了 15 株从犬皮肤分离的马拉色菌 pachydermatis 样本。采用改良肉汤微量稀释（BM）法评估 M. pachydermatis 对伊曲康唑、酮康唑、咪康唑、特比萘芬和 OME 的敏感性。采用 BM 棋盘法评估 OME 单独及与其他抗真菌化合物联合应用对所有分离株的体外疗效。采用部分抑菌浓度指数（FICI）分析法对所得数据进行分析。
抗真菌化合物和 OME 对质控菌株（M. pachydermatis CBS1879）的最小抑菌浓度（MIC）值低于相同药物对临床分离株的 MIC 值。单独用药和联合用药的 MIC 值无显著差异。根据 FICI 分析法分析，OME 与抗真菌化合物之间未观察到相互作用。
大多数马拉色菌菌株对唑类抗真菌药物耐药。OME 对马拉色菌属具有抗真菌作用，甚至对抗真菌耐药株也有良好的效果。抗真菌化合物与 OME 之间未观察到协同作用。
