Schlemmer Karine B, de Jesus Francielli P K, Loreto Erico S, Farias Julia B, Alves Sydney H, Ferreiro Laerte, Santurio Janio M
Programa de Pós-Graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Med Mycol. 2019 Apr 1;57(3):324-327. doi: 10.1093/mmy/myy043.
The yeast Malassezia pachydermatis is a common commensal and occasional opportunistic pathogen of theskin microbiota of animals and humans. In this study, the susceptibility of M. pachydermatis isolates to fluconazole (FLC), itraconazole (ITZ), ketoconazole (KTZ), clotrimazole (CLZ), and miconazole (MCZ) alone and in combination with terbinafine (TRB), nystatin (NYS), and caspofungin (CSP) was evaluated in vitro based on the M27-A3 technique and the checkerboard microdilution method using Sabouraud dextrose broth with 1% tween 80 (SDB). Based on the mean FICI values, the main synergies observed were combinations of ITZ+CSP and CLZ+CSP (55.17%). The most significant combinations deserve in vivo evaluations because might provide effective alternative treatments against M. pachydermatis due to their synergistic interactions.
厚皮马拉色菌是人和动物皮肤微生物群中常见的共生菌，偶尔也会成为机会致病菌。在本研究中，基于M27 - A3技术和棋盘微量稀释法，使用含1%吐温80的沙氏葡萄糖肉汤（SDB），在体外评估了厚皮马拉色菌分离株对氟康唑（FLC）、伊曲康唑（ITZ）、酮康唑（KTZ）、克霉唑（CLZ）和咪康唑（MCZ）单独使用以及与特比萘芬（TRB）、制霉菌素（NYS）和卡泊芬净（CSP）联合使用时的敏感性。基于平均FICI值，观察到的主要协同作用是ITZ + CSP和CLZ + CSP的组合（55.17%）。最显著的组合值得进行体内评估，因为它们的协同相互作用可能为抗厚皮马拉色菌提供有效的替代治疗方法。
