Vahabi Hamed, Liu Jason, Dai Yifan, Joh Daniel Y, Britton Rhett, Heggestad Jacob, Kinnamon David, Rajput Satyam, Chilkoti Ashutosh
Department of Biomedical Engineering, Duke University, Durham, NC, 27705.
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Device. 2023 Jul 21;1(1). doi: 10.1016/j.device.2023.100009. Epub 2023 Jul 11.
We report a simple droplet fluidic point-of-care test (POCT) that uses gravity to manipulate the sequence, timing, and motion of droplets on a surface. To fabricate this POCT, we first developed a surface coating toolbox of nine different coatings with three levels of wettability and three levels of slipperiness that can be independently tailored. We then fabricated a device that has interconnected fluidic elements-pumps, flow resistors and flow guides-on a highly slippery solid surface to precisely control the timing and sequence of motion of multiple droplets and their interactions on the surface. We then used this device to carry out a multi-step enzymatic assay of a clinically relevant analyte-lactate dehydrogenase (LDH)-to demonstrate the application of this technology for point-of-care diagnosis.
我们报告了一种简单的液滴微流即时检测（POCT）方法，该方法利用重力来操控表面上液滴的顺序、时间和运动。为制造这种POCT，我们首先开发了一个包含九种不同涂层的表面涂层工具箱，这些涂层具有三种润湿性水平和三种滑爽性水平，且可独立定制。然后，我们在高度滑爽的固体表面上制造了一种具有相互连接的流体元件（泵、流阻器和流导）的装置，以精确控制多个液滴在表面上的运动时间、顺序及其相互作用。接着，我们使用该装置对临床相关分析物乳酸脱氢酶（LDH）进行了多步酶促测定，以证明该技术在即时诊断中的应用。
