一种重力驱动的微滴式即时检测
A gravity-driven droplet fluidic point-of-care test.
作者信息
Vahabi Hamed, Liu Jason, Dai Yifan, Joh Daniel Y, Britton Rhett, Heggestad Jacob, Kinnamon David, Rajput Satyam, Chilkoti Ashutosh
机构信息
Department of Biomedical Engineering, Duke University, Durham, NC, 27705.
Lead contact:
出版信息
Device. 2023 Jul 21;1(1). doi: 10.1016/j.device.2023.100009. Epub 2023 Jul 11.
We report a simple droplet fluidic point-of-care test (POCT) that uses gravity to manipulate the sequence, timing, and motion of droplets on a surface. To fabricate this POCT, we first developed a surface coating toolbox of nine different coatings with three levels of wettability and three levels of slipperiness that can be independently tailored. We then fabricated a device that has interconnected fluidic elements-pumps, flow resistors and flow guides-on a highly slippery solid surface to precisely control the timing and sequence of motion of multiple droplets and their interactions on the surface. We then used this device to carry out a multi-step enzymatic assay of a clinically relevant analyte-lactate dehydrogenase (LDH)-to demonstrate the application of this technology for point-of-care diagnosis.
我们报告了一种简单的液滴微流即时检测（POCT）方法，该方法利用重力来操控表面上液滴的顺序、时间和运动。为制造这种POCT，我们首先开发了一个包含九种不同涂层的表面涂层工具箱，这些涂层具有三种润湿性水平和三种滑爽性水平，且可独立定制。然后，我们在高度滑爽的固体表面上制造了一种具有相互连接的流体元件（泵、流阻器和流导）的装置，以精确控制多个液滴在表面上的运动时间、顺序及其相互作用。接着，我们使用该装置对临床相关分析物乳酸脱氢酶（LDH）进行了多步酶促测定，以证明该技术在即时诊断中的应用。
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