Materials Genome Institute, Shanghai University, Shanghai 200444, China.
Department of Emergency, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
Nano Lett. 2022 Aug 24;22(16):6833-6840. doi: 10.1021/acs.nanolett.2c01613. Epub 2022 Jul 12.
Limited healthcare capacity highlights the needs of integrated sensing systems for personalized health-monitoring. However, only limited sensors can be employed for point-of-care applications, emphasizing the lack of a generalizable sensing platform. Here, we report a metal organic framework (MOF) ZIF-90-ZnO-MoS nanohybrid-based integrated electrochemical liquid biopsy (ELB) platform capable of direct profiling cancer exosomes from blood. Using a bottom-up approach for sensor design, a series of critical sensing functions is considered and encoded into the MOF material interface by programming the material with different chemical and structural features. The MOF-based ELB platform is able to achieve one-step sensor fabrication, target isolation, nonfouling and high-sensitivity sensing, direct signal transduction, and multiplexed detection. We demonstrated the capability of the designed sensing system on differentiating cancerous groups from healthy controls by analyzing clinical samples from lung cancer patients, providing a generalizable sensing platform.
有限的医疗能力凸显了集成传感系统对于个性化健康监测的需求。然而，用于即时护理应用的传感器数量有限，强调了缺乏通用传感平台的问题。在这里，我们报告了一种基于金属有机骨架（MOF）ZIF-90-ZnO-MoS 纳米杂化的集成电化学液体活检（ELB）平台，该平台能够直接从血液中分析癌症外泌体。我们采用自下而上的传感器设计方法，考虑了一系列关键的传感功能，并通过用不同的化学和结构特征对材料进行编程，将这些功能编码到 MOF 材料界面中。基于 MOF 的 ELB 平台能够实现一步式传感器制造、目标分离、非堵塞和高灵敏度传感、直接信号转导以及多重检测。我们通过分析来自肺癌患者的临床样本，展示了设计的传感系统在区分癌症组和健康对照组方面的能力，为通用传感平台提供了证据。
