肩袖修复联合分泌组-低氧间充质干细胞对肩袖撕裂大鼠TNMD、RUNX2及愈合组织学评分的联合效应
Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX2, and Healing Histology Score in Rotator Cuff Tear Rats.
作者信息
Fredianto Meiky, Herman Herry, Ismiarto Yoyos Dias, Purba Ambrosius, Putra Agung, Hidayah Nurul
机构信息
Doctoral Study Program, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia.
Division of Oncology, Department of Orthopedic Surgery, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia.
出版信息
Arch Bone Jt Surg. 2023;11(10):617-624. doi: 10.22038/ABJS.2023.67933.3218.
OBJECTIVES
In order to treat a rat model of rotator cuff rupture, this work concentrated on the expression of TNMD and RUNX2, followed by rotator cuff repair and secretome-hMSCs.
METHODS
A total of thirty 10-weeks-old male Sprague-Dawley rats were separated into five groups randomly, RC on week 0, lesion treated with a rotator cuff repair and saline (RC + NaCl group, n = 6) for 2 and 8 weeks, and lesion treated with a rotator cuff repair and secretome-hMSCs (RC + secretome-hMSC group, n = 6) for 2 and 8 weeks. The supraspinatus and infraspinatus muscle-tendon units were obtained for histological and biomechanical investigation at 0, 2 and 8 weeks following injury.
RESULTS
The findings showed that, in comparison with the RC + NaCl group, secretome-hMSCs significantly improved tendon repair by upregulating TNMD and RUNX2 expression and histology score.
CONCLUSION
Combining Secretome-hypoxia MSCs with RC healing may help rats with rotator cuff tears.
目的
为了治疗肩袖损伤大鼠模型，本研究聚焦于TNMD和RUNX2的表达，随后进行肩袖修复及分泌组人骨髓间充质干细胞（secretome-hMSCs）治疗。
方法
将30只10周龄雄性Sprague-Dawley大鼠随机分为五组，第0周为肩袖损伤组（RC组），损伤后分别用肩袖修复术加生理盐水处理（RC + NaCl组，n = 6），持续2周和8周；以及用肩袖修复术加分泌组人骨髓间充质干细胞处理（RC + secretome-hMSC组，n = 6），持续2周和8周。在损伤后0、2和8周获取冈上肌和冈下肌肌腱单位进行组织学和生物力学研究。
结果
研究结果显示，与RC + NaCl组相比，分泌组人骨髓间充质干细胞通过上调TNMD和RUNX2表达及组织学评分，显著改善了肌腱修复。
结论
将分泌组低氧人骨髓间充质干细胞与肩袖损伤修复相结合可能有助于治疗肩袖撕裂大鼠。
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