Zaragoza Vargas G, Cacho Calvo J, Martínez-Arce R, Molina Arana D, Ramos Blázquez B, Pérez Abeledo M, Sanz Moreno J C
Gloria Zaragoza Vargas, Servicio de Microbiología. Hospital Universitario de Getafe (Getafe, Madrid)
Rev Esp Quimioter. 2023 Dec;36(6):597-603. doi: 10.37201/req/034.2023. Epub 2023 Oct 24.
The use of conjugate vaccines against Streptococcus pneumoniae originates changes in the invasive pneumococcal disease (IPD). The aim of this study was to in vestigate the evolution of S. pneumoniae serotypes isolated in the Hospital Universitario de Getafe between 2008 and 2022.
313 of S. pneumoniae strains were studied. Serotyping was carried out by latex agglutination (Pneumotest-latex) and the Quellung reaction. In addition, the minimal inhibitory concentration (MIC) was determined against penicillin, erythromycin and levofloxacin by the concentration gradient method (E-test) according the EUCAST breakpoints.
The most frequent serotypes throughout the study period were 8, 3, 19A, 1, 11A and 22F corresponding to 46.6% of the isolates. Along 2008-2012 the serotypes 3, 1, 19A, 7F, 6C and 11A represented altogether 53.6% of the isolates. Between 2013 and 2017 the serotypes 3, 8, 12F, 19A, 22F and 19F grouped 51% of the isolates. During 2018-2022 the serotypes 8, 3, 11A, 15A, 4 and 6C included the 55.5% of the cases. In total 5 strains (1.6%) were penicillin resistant, 64 (20.4%) erythromycin resistant and 11 (3.5%) levofloxacin resistant. The MIC50 and MIC90 levels maintained stables along the time.
The conjugate vaccines use with different serotype coverage conditioned a decrease of the vaccine-included and an increase of non-covered. Despite these changes, the global antimicrobial susceptibility patterns to erythromycin and levofloxacin maintained relatively stables. The resistance a penicillin was low, not finding this type of resistant strains in the last study period.
使用针对肺炎链球菌的结合疫苗引发了侵袭性肺炎球菌疾病（IPD）的变化。本研究的目的是调查2008年至2022年期间在赫塔费大学医院分离出的肺炎链球菌血清型的演变情况。
对313株肺炎链球菌菌株进行了研究。通过乳胶凝集试验（肺炎球菌检测乳胶）和荚膜肿胀反应进行血清分型。此外，根据欧盟药敏试验委员会（EUCAST）的断点，采用浓度梯度法（E试验）测定了对青霉素、红霉素和左氧氟沙星的最低抑菌浓度（MIC）。
在整个研究期间，最常见的血清型为8型、3型、19A 型、1型、11A 型和22F型，占分离株的46.6%。在2008 - 2012年期间，3型、1型、19A 型、7F型、6C型和11A型血清型共占分离株的53.6%。在2013年至2017年期间，3型、8型、12F型、19A 型、22F型和19F型血清型占分离株的51%。在2018 - 2022年期间，8型、3型、11A 型、15A 型、4型和6C型血清型涵盖了55.5%的病例。共有5株（1.6%）对青霉素耐药，64株（20.4%）对红霉素耐药，11株（3.5%）对左氧氟沙星耐药。MIC50和MIC90水平随时间保持稳定。
使用具有不同血清型覆盖范围的结合疫苗导致了疫苗涵盖血清型的减少和未涵盖血清型的增加。尽管有这些变化，但对红霉素和左氧氟沙星的总体抗菌药敏模式保持相对稳定。对青霉素的耐药性较低，在最后一个研究期间未发现此类耐药菌株。
