School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan City, China.
School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan City, China.
Appl Biochem Biotechnol. 2024 Jul;196(7):4067-4087. doi: 10.1007/s12010-023-04744-y. Epub 2023 Oct 24.
This study investigated the chemical and volatile characteristics of sea buckthorn fruits from three different regions in China. The chemical composition of the volatile oil was determined by using a non-targeted gas chromatography and mass spectrometry (GC/MS) method and the differences in chemical composition among the three producing areas were compared by heatmap providing a visual basis for researchers. A total of 93 compounds were identified, including 52 compounds from the Northeast China, 51 from the Xinjiang region, and 37 from Inner Mongolia region. Then, the in vitro antioxidant activity of sea buckthorn fruit oil was measured using DPPH, ABTS, and SOD inhibition tests, and the results showed that sea buckthorn fruit oil in northeast China was the strongest antioxidant, followed by Inner Mongolia and Xinjiang. The results of the CCK-8 experiment indicated that within the tested concentration, there is no cell cytotoxicity of the essential oil in human umbilical vein endothelial cells (HUVECs) cells. The results could supply reference to distinguish sea buckthorn fruit from different production areas and, meanwhile, clarify the activity and safety of sea buckthorn oil.
本研究调查了中国三个不同地区沙棘果实的化学和挥发性特征。采用非靶向气相色谱-质谱联用（GC/MS）方法确定挥发油的化学成分，并通过热图比较三个产地化学成分的差异，为研究人员提供直观的依据。共鉴定出 93 种化合物，其中东北 52 种，新疆 51 种，内蒙古 37 种。然后，采用 DPPH、ABTS 和 SOD 抑制试验测定沙棘果油的体外抗氧化活性，结果表明东北沙棘果油的抗氧化能力最强，其次是内蒙古和新疆。CCK-8 实验结果表明，在所测试的浓度范围内，人脐静脉内皮细胞（HUVECs）中的精油没有细胞毒性。该结果可为鉴别不同产地的沙棘果实提供参考，同时阐明沙棘油的活性和安全性。
