University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-Napoca, Romania.
Phytochem Anal. 2013 Jul-Aug;24(4):319-28. doi: 10.1002/pca.2413. Epub 2013 Jan 15.
The health benefits of sea buckthorn (Hippophae rhamnoides L.) are well documented due to its rich content in bioactive phytochemicals (pigments, phenolics and vitamins) as well as volatiles responsible for specific flavours and bacteriostatic action. The volatile compounds are good biomarkers of berry freshness, quality and authenticity.
To develop a fast and efficient GC-MS method including a minimal sample preparation technique (in-tube extraction, ITEX) for the discrimination of sea buckthorn varieties based on their chromatographic volatile fingerprint.
Twelve sea buckthorn varieties (wild and cultivated) were collected from forestry departments and experimental fields, respectively. The extraction of volatile compounds was performed using the ITEX technique whereas separation and identification was performed using a GC-MS QP-2010. Principal component analysis (PCA) was applied to discriminate the differences among sample composition.
Using GC-MS analysis, from the headspace of sea buckthorn samples, 46 volatile compounds were separated with 43 being identified. The most abundant derivatives were ethyl esters of 2-methylbutanoic acid, 3-methylbutanoic acid, hexanoic acid, octanoic acid and butanoic acid, as well as 3-methylbutyl 3-methylbutanoate, 3-methylbutyl 2-methylbutanoate and benzoic acid ethyl ester (over 80% of all volatile compounds). Principal component analysis showed that the first two components explain 79% of data variance, demonstrating a good discrimination between samples.
A reliable, fast and eco-friendly ITEX/GC-MS method was applied to fingerprint the volatile profile and to discriminate between wild and cultivated sea buckthorn berries originating from the Carpathians, with relevance to food science and technology.
沙棘（Hippophae rhamnoides L.）由于其富含生物活性植物化学物质（色素、酚类和维生素）以及负责特定风味和抑菌作用的挥发性物质，其对健康的益处得到了充分证明。挥发性化合物是浆果新鲜度、质量和真实性的良好生物标志物。
开发一种快速有效的 GC-MS 方法，包括一种最小样品制备技术（管内提取，ITEX），用于根据浆果的色谱挥发性指纹鉴别沙棘品种。
从林业局和实验田分别收集了 12 种沙棘（野生和栽培）品种。采用 ITEX 技术提取挥发性化合物，采用 GC-MS QP-2010 进行分离和鉴定。应用主成分分析（PCA）来区分样品组成的差异。
使用 GC-MS 分析，从沙棘样品的顶空部分分离出 46 种挥发性化合物，其中 43 种得到鉴定。最丰富的衍生物是 2-甲基丁酸、3-甲基丁酸、己酸、辛酸和丁酸的乙酯，以及 3-甲基丁基 3-甲基丁酸酯、3-甲基丁基 2-甲基丁酸酯和苯甲酸乙酯（超过所有挥发性化合物的 80%）。主成分分析表明，前两个成分解释了 79%的数据方差，表明样品之间的区分良好。
应用可靠、快速和环保的 ITEX/GC-MS 方法对指纹图谱的挥发性特征进行了研究，并对源自喀尔巴阡山脉的野生和栽培沙棘浆果进行了区分，这与食品科学和技术有关。
