State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China.
Cell Rep. 2023 Nov 28;42(11):113290. doi: 10.1016/j.celrep.2023.113290. Epub 2023 Oct 23.
Sclerotinia sclerotiorum and Botrytis cinerea are necrotrophic plant-pathogenic fungi, causing substantial economic losses on many crops. So far, resistant cultivars against these pathogens are unavailable in most crops. Here, we show that the serine protease CmSp1 of Coniothyrium minitans, a well-characterized mycoparasite of S. sclerotiorum, contributed to suppressing the petal-mediated infection by S. sclerotiorum in rapeseed. Application of recombinant CmSp1 proteins facilitates the bulk degradation of S. sclerotiorum proteins and inhibits spore germination and hyphal growth of S. sclerotiorum and B. cinerea, thereby preventing the development of both diseases. Stable transgenic rapeseed plants with tissue-specific expression of CmSp1 in flower petals inhibit the petal-mediated infection by both S. sclerotiorum and B. cinerea, and resulting transgenic plants have no adverse effect on other agronomic traits. Thus, our findings provide a novel mechanism by which a mycoparasite inhibits fungal pathogens and an environmentally friendly disease management strategy.
核盘菌和灰葡萄孢是两种专性坏死型植物病原菌，会给许多作物造成巨大的经济损失。迄今为止，大多数作物都缺乏针对这些病原菌的抗性品种。在这里，我们发现小球腔菌 CmSp1 这种被深入研究的核盘菌的真菌寄生菌，有助于抑制油菜花瓣介导的核盘菌侵染。重组 CmSp1 蛋白的应用促进了核盘菌蛋白的大量降解，并抑制了核盘菌和灰葡萄孢的孢子萌发和菌丝生长，从而防止了这两种病害的发生。在油菜花瓣中组织特异性表达 CmSp1 的稳定转基因油菜植株抑制了花瓣介导的核盘菌和灰葡萄孢侵染，并且转基因植株对其他农艺性状没有不良影响。因此，我们的研究结果为真菌寄生菌抑制真菌病原菌提供了一种新的机制，并为环境友好的病害管理策略提供了新的思路。
