Yang Jing, He Helen, Dong Shijie, Lv Jing, Cheng Lili, Yu Qiaoqiao, Kang Le, Guo Xiaojiao
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Science, Beijing 100101, China.
University of Chinese Academy of Science, Beijing 100101, China.
Sci Adv. 2025 Sep 12;11(37):eads1352. doi: 10.1126/sciadv.ads1352. Epub 2025 Sep 10.
Insects, unlike vertebrates, use heteromeric complexes of odorant receptors and co-receptors for olfactory signal transduction. However, the secondary messengers involved in this process are largely unknown. Here, we use the olfactory signal transduction of the aggregation pheromone 4-vinylanisole (4VA) as a model to address this question. When locusts detect 4VA, the pheromone is transported by OBP10 and OBP13 to the OR35-Orco receptor complex, thereby activating downstream pathways in the antenna. A pivotal downstream molecule, the lipid-binding protein Clvs2, facilitates phosphatidylinositol 4,5-bisphosphate transportation across the cytolemma, providing more substrates for inositol trisphosphate (IP) production. PLCe1, a biosynthetic enzyme, boosts IP levels in the antennal lobe of the brain. IP is responsible for converting chemical signals from the antenna into neural signals, confirming IP as a secondary messenger in olfaction perception instead of GPCR in locusts. These findings elucidate the universal function of IP in olfactory signal perception, shedding light on the key nodes of insect olfactory signal transduction.
与脊椎动物不同，昆虫利用气味受体和共受体的异源复合物进行嗅觉信号转导。然而，参与这一过程的第二信使在很大程度上尚不清楚。在这里，我们以聚集信息素4-乙烯基苯甲醚（4VA）的嗅觉信号转导为模型来解决这个问题。当蝗虫检测到4VA时，该信息素由OBP10和OBP13转运至OR35-Orco受体复合物，从而激活触角中的下游通路。一个关键的下游分子，即脂质结合蛋白Clvs2，促进磷脂酰肌醇4,5-二磷酸跨细胞膜运输，为肌醇三磷酸（IP）的产生提供更多底物。生物合成酶PLCe1提高了脑触角叶中的IP水平。IP负责将来自触角的化学信号转化为神经信号，证实IP是蝗虫嗅觉感知中的第二信使，而非GPCR。这些发现阐明了IP在嗅觉信号感知中的普遍功能，揭示了昆虫嗅觉信号转导的关键节点。
