Center for Integrated Medical Research, Keio University School of Medicine, Shinjuku-ku, Tokyo, 160-8582, Japan.
Proteome Homeostasis Research Unit, RIKEN Center for Integrative Medical Sciences, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Sci Rep. 2023 Oct 24;13(1):18191. doi: 10.1038/s41598-023-45570-w.
Brain aging causes a progressive decline in functional capacity and is a strong risk factor for dementias such as Alzheimer's disease. To characterize age-related proteomic changes in the brain, we used quantitative proteomics to examine brain tissues, cortex and hippocampus, of mice at three age points (3, 15, and 24 months old), and quantified more than 7000 proteins in total with high reproducibility. We found that many of the proteins upregulated with age were extracellular proteins, such as extracellular matrix proteins and secreted proteins, associated with glial cells. On the other hand, many of the significantly downregulated proteins were associated with synapses, particularly postsynaptic density, specifically in the cortex but not in the hippocampus. Our datasets will be helpful as resources for understanding the molecular basis of brain aging.
大脑衰老导致功能能力逐渐下降，是阿尔茨海默病等痴呆症的一个重要危险因素。为了描述大脑中与年龄相关的蛋白质组变化，我们使用定量蛋白质组学方法检测了三个年龄点（3、15 和 24 个月）的小鼠脑组织、皮层和海马组织，总共定量了超过 7000 种具有高重复性的蛋白质。我们发现，许多随年龄上调的蛋白质是细胞外蛋白质，如细胞外基质蛋白和分泌蛋白，与神经胶质细胞有关。另一方面，许多显著下调的蛋白质与突触有关，特别是突触后密度，特别是在皮层而不是海马体中。我们的数据集将有助于理解大脑衰老的分子基础。
