Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kawaramachi Hirokoji Kamigyo-ku, Kyoto, 602-8566, Japan.
Department of Gastroenterology and Hepatology, Japanese Red Cross Society Kyoto Daiichi Hospital, Kyoto, Japan.
Dig Dis Sci. 2023 Dec;68(12):4398-4406. doi: 10.1007/s10620-023-08154-2. Epub 2023 Oct 24.
Helicobacter pylori (H. pylori) is widely recognized as a definite carcinogen in gastric cancer (GC). Although H. pylori eradication reduces the risk of GC, GC recurrence has been detected even after successful H. pylori eradication. Recently, the analysis of gut microbiota was reported.
This study aimed to evaluate the correlation between gastric mucosa-associated microbiota (G-MAM) and early gastric cancer (EGC) after successful H. pylori eradication.
In this pilot study, G-MAM were collected during the esophagogastroduodenoscopy of 17 patients, receiving H. pylori eradication therapy at least 5 years ago. The patients were divided into those with EGC (the EGC group, 8 patients) and those without EGC (the NGC group, 9 patients). Microbial samples in the greater curvature of the pyloric site were obtained using an endoscopic cytology brush, and the G-MAM profiles of each sample were analyzed using 16S rRNA V3-V4 gene sequencing.
Between the two groups, there was no significant difference in the median age, sex, median period after successful eradication of H. pylori, the α diversity, and the average abundance at the phylum level. At the genus level, the average abundance of Unclassified Oxalobacteraceae, Capnocytophaga, and Haemophilus was significantly lower in the EGC group than in the NGC group (0.89 vs. 0.14%, P < 0.01, 0.28 vs. 0.00%, P < 0.01 and 5.84 vs. 2.16%, P = 0.034, respectively).
We demonstrated alternations in the profiles of G-MAM between the two groups. Our results suggest that G-MAM may influence carcinogenesis after successful H. pylori eradication.
幽门螺杆菌（H. pylori）被广泛认为是胃癌（GC）的明确致癌物。尽管 H. pylori 根除可降低 GC 的风险，但即使在成功根除 H. pylori 后，也已检测到 GC 复发。最近，有报道对肠道微生物群进行了分析。
本研究旨在评估成功根除 H. pylori 后胃黏膜相关微生物群（G-MAM）与早期胃癌（EGC）之间的相关性。
在这项初步研究中，收集了 17 名患者的 G-MAM，这些患者在至少 5 年前接受了 H. pylori 根除治疗。将患者分为患有 EGC（EGC 组，8 例）和未患有 EGC（NGC 组，9 例）的患者。使用内镜细胞学刷从幽门部位的大曲率处获取微生物样本，并使用 16S rRNA V3-V4 基因测序分析每个样本的 G-MAM 图谱。
两组间的中位年龄、性别、成功根除 H. pylori 后的中位时间、α多样性以及门水平的平均丰度均无显著差异。在属水平上，EGC 组的未分类 Oxalobacteraceae、Capnocytophaga 和 Haemophilus 的平均丰度明显低于 NGC 组（0.89%比 0.14%，P<0.01，0.28%比 0.00%，P<0.01 和 5.84%比 2.16%，P=0.034）。
我们证明了两组之间 G-MAM 谱的变化。我们的结果表明，G-MAM 可能会影响成功根除 H. pylori 后的致癌作用。
