Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Digestion. 2020;101(2):107-120. doi: 10.1159/000496102. Epub 2019 Feb 5.
The importance of microbiota infiltrating the gut mucus layer has been reported in the pathogenesis of various gastrointestinal and systemic diseases. However, little is known about the mucosa-associated microbiota (MAM) in healthy subjects. The present study aimed to clarify the characteristics of the gastrointestinal MAM from the oral cavity to the rectum in healthy Japanese subjects.
Seventeen healthy subjects were enrolled. In this study, 5 mucosa samples from the upper gut (intraoral, mid-esophagus, gastric corpus, gastric antrum, and duodenum) and 7 from the lower gut (ileum, cecum, ascending colon, transverse colon, descending colon, sigmoid colon, and rectum) were collected with a brush under endoscopic examination. MAM profiles of each sample were analyzed by 16S-rRNA V3-V4 gene sequences.
Collecting mucosa samples by brushing provided sufficient material for MAM profiling without causing adverse effects. The upper and lower gut MAM profiles differed significantly (p < 0.0001). In the upper and lower gut, the intra- and inter-individual MAM profiles were significantly different (p = 0.0008 and p < 0.0001 respectively).
The MAM profiles of the upper and lower gut were significantly different. The inter-individual differences in MAM were remarkable compared to the intra-individual differences.
微生物群渗透到肠道黏液层在各种胃肠道和系统性疾病的发病机制中具有重要意义。然而，对于健康受试者的黏膜相关微生物群（MAM）知之甚少。本研究旨在阐明健康日本受试者从口腔到直肠的胃肠道 MAM 的特征。
纳入了 17 名健康受试者。在这项研究中，通过内镜检查用刷子采集了 5 个上消化道黏膜样本（口腔内、食管中段、胃体、胃窦和十二指肠）和 7 个下消化道黏膜样本（回肠、盲肠、升结肠、横结肠、降结肠、乙状结肠和直肠）。通过 16S-rRNA V3-V4 基因序列分析每个样本的 MAM 图谱。
用刷子采集黏膜样本为 MAM 分析提供了足够的材料，而不会引起不良反应。上消化道和下消化道的 MAM 图谱有显著差异（p<0.0001）。在上消化道和下消化道中，个体内和个体间的 MAM 图谱差异显著（p=0.0008 和 p<0.0001 分别）。
上消化道和下消化道的 MAM 图谱有显著差异。与个体内差异相比，MAM 的个体间差异更为显著。
