Setor de Imunologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, Brazil.
Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, Brazil.
Parasitol Res. 2023 Dec;122(12):3147-3158. doi: 10.1007/s00436-023-08005-z. Epub 2023 Oct 25.
Anti-Ascaris lumbricoides (Asc) IgE and IgG can immunomodulate the allergy; however, the influence of these isotypes has not been investigated in the giardiasis and allergy. Therefore, the frequency of respiratory allergy (RA) symptoms in Giardia lamblia-infected children, with or without anti-Asc IgE, IgG1, or IgG4 and Th1, Th2/Treg, and Th17 cytokine production, was evaluated. We performed a case-control study with children aged 2-10 years old selected by questionnaire and stool exams to form the groups: infected or uninfected with RA (G-RA, n = 55; nG-RA, n = 43); infected and uninfected without RA (G-nRA, n = 59; nG-nRA, n = 54). We performed blood leukocyte counts and in vitro culture. Cytokine levels in the supernatants (CBA), serum total IgE and anti-Asc IgE (ImmunoCAP), IgG1, IgG4, and total IgA (ELISA) were measured. Infection was not associated with allergy. Infected children showed increased levels of anti-Asc IgG1, IL-2, IFN-γ, IL-4, and IL-10. There was a lower frequency of allergy-related symptoms in anti-Asc IgG1-positive children than IgG1-negative (OR = 0.38; CI = 0.17-0.90, p = 0.027) and few eosinophils in G-RA than in G-nRA and more in G-nRA than in nG-nRA, whereas TNF-α levels were higher in the G-RA than in the nG-nRA group. For infected and positive anti-Asc IgG1, there was higher TNF-α and IL-10 production than G/-IgG1. IL-10 levels were lower in nG/ + IgG1 than in infected or non-infected, and both were negative for anti-Asc IgG1. Th1/Th2/IL-10 profiles were stimulated in the infected patients, and in those with circulating anti-Asc IgG1, the TNF-α production was strengthened with a lower risk for respiratory allergy symptoms.
抗蛔虫 IgE 和 IgG 可调节过敏反应；然而，这些同种型在贾第虫病和过敏中的影响尚未被研究。因此，我们评估了患有呼吸道过敏（RA）症状和无呼吸道过敏症状的蓝氏贾第鞭毛虫感染儿童，以及具有或不具有抗蛔虫 IgE、IgG1 或 IgG4 以及 Th1、Th2/Treg 和 Th17 细胞因子产生的频率。我们进行了一项病例对照研究，通过问卷调查和粪便检查选择 2-10 岁的儿童，形成以下组：感染或未感染 RA（G-RA，n=55；nG-RA，n=43）；感染但未感染 RA（G-nRA，n=59；nG-nRA，n=54）。我们进行了白细胞计数和体外培养。测量上清液中的细胞因子水平（CBA）、血清总 IgE 和抗蛔虫 IgE（ImmunoCAP）、IgG1、IgG4 和总 IgA（ELISA）。感染与过敏无关。感染的儿童显示抗蛔虫 IgG1、IL-2、IFN-γ、IL-4 和 IL-10 水平升高。与 IgG1 阴性儿童相比，抗蛔虫 IgG1 阳性儿童发生与过敏相关的症状的频率较低（OR=0.38；CI=0.17-0.90，p=0.027），G-RA 中的嗜酸性粒细胞比 G-nRA 少，G-nRA 中的嗜酸性粒细胞比 nG-nRA 多，而 G-RA 中的 TNF-α水平高于 nG-nRA 组。对于感染和阳性抗蛔虫 IgG1，TNF-α和 IL-10 的产生高于 G/-IgG1。nG/ + IgG1 的 IL-10 水平低于感染或未感染的水平，并且两者均未检测到抗蛔虫 IgG1。感染患者中存在 Th1/Th2/IL-10 谱刺激，并且在具有循环抗蛔虫 IgG1 的患者中，TNF-α的产生增强，患呼吸道过敏症状的风险降低。
