Yang Ping, Zhong JianBo, Zhao XingYun, Ali Kamran, Wu Liming, Bu ZhangYu
Department of Dermatology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Department of Oncology, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu, Zhejiang, People's Republic of China.
Clin Cosmet Investig Dermatol. 2023 Oct 19;16:2957-2963. doi: 10.2147/CCID.S428587. eCollection 2023.
Hypertrophic scars, commonly occurring after trauma or surgery in critical areas like the head and joints, pose significant challenges to both physical and mental health due to their impact on skin function and aesthetics. While the complex pathogenesis involves fibroblast activation and collagen deposition, effective treatments are lacking, underscoring the importance of exploring pathogenesis and targeted therapies. Hesperidin, a bioactive compound in citrus fruits with diverse health benefits, including anti-fibrotic and anti-angiogenic effects, is the focus of this study with the aim of investigating its impact on hypertrophic scar formation, given its potential to promote blood flow and improve skin microcirculation.
This study aimed to observe the inhibitory effect of topical hesperidin on hypertrophic scars in rabbits.
A total of 8 healthy adult New Zealand white rabbits were used to establish a rabbit hypertrophic scarring animal model. Five wounds were created on each rabbit's two ears, with three wounds on the left ear (groups A, B, and C) and two wounds on the right ear (groups D and E). After six days of wound drying postoperatively, the wounds were locally treated with medication as followed: group A, 0.1% hesperidin; group B, 1% hesperidin; group C, 1% triamcinolone acetonide; group D, Vaseline; and group E, blank control without any medication. After 28 days, the scar tissue samples were collected for histological examination.
The results showed that the scar texture of groups B and C was softer and lighter in color, and the number of fibroblasts, capillaries, and inflammatory cells in the tissue was significantly less than those in the other three groups. The hypertrophic scar indices of groups B and C were significantly smaller than those of groups A, D, and E, and the difference was statistically significant (P < 0.05). There were no significant difference between groups B and C.
Topical application of hesperidin demonstrated promising potential for reducing hypertrophic scar formation in rabbits.
肥厚性瘢痕常见于头部和关节等关键部位的创伤或手术后，因其对皮肤功能和美观的影响，对身心健康构成重大挑战。虽然其复杂的发病机制涉及成纤维细胞活化和胶原蛋白沉积，但缺乏有效的治疗方法，这凸显了探索发病机制和靶向治疗的重要性。橙皮苷是柑橘类水果中的一种生物活性化合物，具有多种健康益处，包括抗纤维化和抗血管生成作用，鉴于其促进血流和改善皮肤微循环的潜力，本研究将其作为重点，旨在研究其对肥厚性瘢痕形成的影响。
本研究旨在观察局部应用橙皮苷对兔肥厚性瘢痕的抑制作用。
共选用8只健康成年新西兰白兔建立兔肥厚性瘢痕动物模型。在每只兔子的两只耳朵上制造5个伤口，左耳3个伤口（A、B、C组），右耳2个伤口（D、E组）。术后伤口干燥6天后，对伤口进行如下局部用药：A组，0.1%橙皮苷；B组，1%橙皮苷；C组，1%曲安奈德；D组，凡士林；E组，无任何药物的空白对照。28天后，收集瘢痕组织样本进行组织学检查。
结果显示，B组和C组瘢痕质地较软，颜色较浅，组织中成纤维细胞、毛细血管和炎性细胞数量明显少于其他三组。B组和C组的肥厚性瘢痕指数明显小于A、D、E组，差异具有统计学意义（P<0.05）。B组和C组之间无显著差异。
局部应用橙皮苷在减少兔肥厚性瘢痕形成方面显示出有前景的潜力。
