Bilska Bernadetta, Damulewicz Milena, Abaquita Terence Al L, Pyza Elzbieta
Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Cracow, Poland.
Front Cell Neurosci. 2023 Oct 9;17:1239101. doi: 10.3389/fncel.2023.1239101. eCollection 2023.
Heme oxygenase (HO) has been shown to control various cellular processes in both mammals and . Here, we investigated how changes in HO levels in neurons and glial cells during development affect adult flies, by using the TARGET system to manipulate the expression of the gene. The obtained data showed differences in adult survival, maximum lifespan, climbing, locomotor activity, and sleep, which depended on the level of HO (after up-regulation or downregulation), the timing of expression (chronic or at specific developmental stages), cell types (neurons or glia), sex (males or females), and age of flies. In addition to the effects of changing the mRNA level of the CNC factor gene (NRF2 homolog in mammals and master regulator of HO), were also examined to compare with those observed after changing expression. We showed that HO levels in neurons and glia must be maintained at an appropriate physiological level during development to ensure the well-being of adults. We also found that the downregulation of in either neurons or glia in the brain is compensated by expressed in the retina.
血红素加氧酶（HO）已被证明可控制哺乳动物和[此处原文缺失相关内容]中的各种细胞过程。在这里，我们通过使用TARGET系统操纵[此处原文缺失相关基因名称]基因的表达，研究了发育过程中神经元和神经胶质细胞中HO水平的变化如何影响成年果蝇。获得的数据显示，成年果蝇在存活、最大寿命、攀爬、运动活动和睡眠方面存在差异，这些差异取决于HO的水平（上调或下调后）、表达时间（长期或在特定发育阶段）、细胞类型（神经元或神经胶质细胞）、性别（雄性或雌性）以及果蝇的年龄。除了[此处原文缺失相关内容]，还研究了改变CNC因子基因（哺乳动物中的NRF2同源物和HO的主要调节因子）mRNA水平的影响，以与改变[此处原文缺失相关基因名称]表达后观察到的影响进行比较。我们表明，发育过程中神经元和神经胶质细胞中的HO水平必须维持在适当的生理水平，以确保成年果蝇的健康。我们还发现，大脑中神经元或神经胶质细胞中[此处原文缺失相关基因名称]的下调可由视网膜中表达的[此处原文缺失相关内容]来补偿。
