Wang Lijuan, Wu Zhihao, Liu Mengxia, Liu Wei, Zhao Wenxi, Liu Hongjun, You Feng
CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong, China.
PeerJ. 2018 Jun 25;6:e5013. doi: 10.7717/peerj.5013. eCollection 2018.
Rongcheng Bay is a coastal bay of the Northern Yellow Sea, China. To investigate and monitor the fish resources in Rongcheng Bay, 187 specimens from 41 different species belonging to 28 families in nine orders were DNA-barcoded using the mitochondrial cytochrome c oxidase subunit I gene (). Most of the fish species could be discriminated using this COI sequence with the exception of and The average GC% content of the 41 fish species was 47.3%. The average Kimura 2-parameter genetic distances within the species, genera, families, and orders were 0.21%, 5.28%, 21.30%, and 23.63%, respectively. Our results confirmed that the use of combined morphological and DNA barcoding identification methods facilitated fish species identification in Rongcheng Bay, and also established a reliable DNA barcode reference library for these fish. DNA barcodes will contribute to future efforts to achieve better monitoring, conservation, and management of fisheries in this area.
荣成湾是中国黄海北部的一个沿岸海湾。为了调查和监测荣成湾的鱼类资源，利用线粒体细胞色素c氧化酶亚基I基因对9目28科41个不同物种的187个标本进行了DNA条形码分析。除了[具体物种未给出]和[具体物种未给出]外，大多数鱼类物种都可以通过该COI序列进行鉴别。41种鱼类的平均GC%含量为47.3%。种内、属内、科内和目内的平均Kimura 2-参数遗传距离分别为0.21%、5.28%、21.30%和23.63%。我们的结果证实，形态学和DNA条形码鉴定方法相结合有助于荣成湾鱼类物种的鉴定，同时也为这些鱼类建立了可靠的DNA条形码参考库。DNA条形码将有助于未来更好地监测、保护和管理该地区的渔业资源。
