State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, P. R. China.
Anal Chem. 2023 Nov 7;95(44):16225-16233. doi: 10.1021/acs.analchem.3c03020. Epub 2023 Oct 25.
Compared with the accuracy of a single signal and the limitation of environmental applicability, the application value of dual-mode detection is gradually increasing. To this end, based on the photothermal effect of Ag/Co embedded N-rich mesoporous carbon nanomaterials (AgCo@NC NPs), we designed a dual-mode signal response system for the detection of α-fetoprotein (AFP). First, AgCo@NC NPs act as a photothermal immunoprobe that converts light energy into heat driven by a near-infrared (NIR) laser and obtains temperature changes corresponding to the analyte concentration on a hand-held thermal imager. In addition, this temperature recognition system can significantly improve the efficiency of Fenton-like reactions. AgCo@NC NPs act as peroxidase mimics to initiate the generation of poly -isopropylacrylamide (PNIPAM, resistance enhancer) by cascade catalysis and the degradation of methylene blue (MB), thus enabling electrochemical testing. The dual-mode assay ranges from 0.01 to 100 and 0.001-10 ng/mL, with lower limits of detection (LOD) of 3.2 and 0.089 pg/mL, respectively, and combines visualization, portability, and high efficiency, opening new avenues for future clinical diagnostics and inhibitor studies.
与单一信号的准确性和环境适用性的限制相比，双模式检测的应用价值逐渐增加。为此，基于 Ag/Co 嵌入富氮介孔碳纳米材料（AgCo@NC NPs）的光热效应，我们设计了一种用于检测甲胎蛋白（AFP）的双模式信号响应系统。首先，AgCo@NC NPs 作为光热免疫探针，在近红外（NIR）激光的驱动下将光能转化为热能，并在手持式热像仪上获得与分析物浓度相对应的温度变化。此外，这种温度识别系统可以显著提高类芬顿反应的效率。AgCo@NC NPs 作为过氧化物酶模拟物，通过级联催化引发聚异丙基丙烯酰胺（PNIPAM，增强阻力剂）的生成和亚甲基蓝（MB）的降解，从而实现电化学测试。双模式测定范围为 0.01 至 100 和 0.001 至 10 ng/mL，检测限（LOD）分别为 3.2 和 0.089 pg/mL，结合可视化、便携性和高效率，为未来的临床诊断和抑制剂研究开辟了新途径。
