Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Tissue Cell. 2023 Dec;85:102248. doi: 10.1016/j.tice.2023.102248. Epub 2023 Oct 14.
Sperm quality is a complex index used to evaluate the fertility potential of men. The long non-coding RNA (lncRNA) MALAT1 participate in sperm development and HOTAIR have critical roles in the regulation of oxidative stress responses. This study aimed to evaluate the relationship of lncRNA MALAT1 and HOTAIR expression with sperm parameters, DNA fragmentation and malondialdehyde (MDA)levels in sperm fertility.
In this experimental study, semen samples (n = 30 fertile, n = 30 infertile) men were collected and evaluated for sperm parameters by computer-aided sperm analysis(CASA). Sperm DNA integrity quality was assessed by the Acridine orange(AO) test. MDA levels were determined by the Thiobarbituric acid reaction method. The expression of MALAT1 and HOTAIR was detected by RT-PCR.
We observed a decreased level of MALAT1and HOTAIR expression in the infertile men (p < 0.001). The relative expression level of MALAT1and HOTAIR showed a positive correlation with motility and morphology (p < 0.001). Subsequently, we found the DNA damage and MDA levels was negatively correlated with expression level of genes of sperm (p < 0.001).
In this study the low expression of MALATI and HOTAIR resulted in the high level of MDA, DNA damage, and reduced motility of sperm. This study suggests the therapeutic opportunities in respect to MALATI and HOTAIR expression in the sperm function.
精子质量是评估男性生育能力的一个复杂指标。长链非编码 RNA（lncRNA）MALAT1 参与精子发生，HOTAIR 在调节氧化应激反应中起着关键作用。本研究旨在评估 lncRNA MALAT1 和 HOTAIR 表达与精子参数、精子 DNA 碎片化和精子中丙二醛（MDA）水平的关系。
在这项实验研究中，收集了 30 名生育能力正常男性和 30 名不育男性的精液样本，并通过计算机辅助精子分析（CASA）评估精子参数。通过吖啶橙（AO）试验评估精子 DNA 完整性质量。通过硫代巴比妥酸反应法测定 MDA 水平。通过 RT-PCR 检测 MALAT1 和 HOTAIR 的表达。
我们观察到不育男性 MALAT1 和 HOTAIR 的表达水平降低（p<0.001）。MALAT1 和 HOTAIR 的相对表达水平与活力和形态呈正相关（p<0.001）。随后，我们发现 DNA 损伤和 MDA 水平与精子基因的表达水平呈负相关（p<0.001）。
在这项研究中，MALATI 和 HOTAIR 的低表达导致 MDA、DNA 损伤和精子活力降低。本研究表明，在精子功能方面，MALATI 和 HOTAIR 表达具有治疗潜力。
