Division of Basic Science & Translational Research, Department of Obstetrics & Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas, USA.
Department of Physiology, College of Medicine, University of the Philippines Manila, Manila, Philippines.
Am J Reprod Immunol. 2023 Nov;90(5):e13784. doi: 10.1111/aji.13784.
An intact cervix is a barrier that prevents pathogenic bacteria from invading the uterine and amniotic cavity during pregnancy. Its disruption is associated with ascending infection and adverse pregnancy outcomes. This study analyzed the effects of bisphenol A (BPA), a chemical used in plastics manufacturing, on cell death and inflammation in cervical epithelial cells.
Ectocervical epithelial (ecto) and endocervical epithelial (endo) cells were treated with 100 ng/mL and 300 ng/mL of BPA for 48 h. The cells were subjected to flow cytometry using annexin V and propidium iodide to determine apoptosis and necrosis, cell cycle analysis, and ELISA to determine the levels of inflammatory cytokines (IL-6, IL-8, and IL-10).
Low-dose and high-dose BPA significantly increased the live ecto cell population dose-dependently. BPA did not have any noticeable effect on cell cycle progression in either cell type. BPA treatment also decreased the apoptotic ecto and endo cell population dose-dependently. Lastly, high dose BPA significantly increased IL-6 in ecto and endo cells. However, IL-8 and IL-10 were not affected by BPA treatments.
Chemical exposure damage to the cervix can lead to adverse pregnancy outcomes. Our study showed that the BPA concentrations reported in pregnant subjects do not induce cervical cell toxicity . The decrease in apoptosis and increase in live cells may be a compensatory mechanism to preserve the integrity of the cervical epithelial layer.
在怀孕期间，完整的宫颈是阻止致病菌侵入子宫和羊膜腔的屏障。其破坏与上行感染和不良妊娠结局有关。本研究分析了双酚 A（BPA）这种用于制造塑料的化学物质对宫颈上皮细胞死亡和炎症的影响。
用 100ng/ml 和 300ng/ml 的 BPA 处理宫颈外上皮（ecto）和宫颈内上皮（endo）细胞 48 小时。用 Annexin V 和碘化丙啶通过流式细胞术检测细胞凋亡和坏死，细胞周期分析和 ELISA 检测炎症细胞因子（IL-6、IL-8 和 IL-10）水平。
低剂量和高剂量 BPA 显著增加活 ecto 细胞群体，呈剂量依赖性。BPA 对两种细胞类型的细胞周期进程均无明显影响。BPA 处理还使凋亡的 ecto 和 endo 细胞群体呈剂量依赖性减少。最后，高剂量 BPA 显著增加 ecto 和 endo 细胞中的 IL-6。然而，BPA 处理并不影响 IL-8 和 IL-10。
化学物质暴露对宫颈的损伤可导致不良妊娠结局。我们的研究表明，在妊娠受试者中报告的 BPA 浓度不会引起宫颈细胞毒性。凋亡减少和活细胞增加可能是维持宫颈上皮层完整性的补偿机制。
