利用红色发光碳纳米点作为荧光生物标志物追踪超分辨结构的线粒体。

Tracking the super resolved structure of mitochondria using red emissive carbon nanodots as a fluorescent biomarker.

机构信息

School of Chemical Sciences, Indian Institute of Technology (IIT), Mandi, H.P.-175005, India.

School of Biosciences and Bioengineering, Indian Institute of Technology (IIT), Mandi, H.P.-175005, India.

出版信息

Chem Commun (Camb). 2023 Nov 9;59(90):13454-13457. doi: 10.1039/d3cc03390b.

DOI:10.1039/d3cc03390b

PMID:37882736

Abstract

Herein, we report new red emissive highly photostable and water-soluble carbon nanodots (TPP CNDs) to visualize mitochondrial dynamics using super-resolution radial fluctuations (SRRF) microscopy. The TPP CNDs were synthesized in a one-step method, using 3-(carboxypropyl)triphenylphosphonium bromide (TPP) and -phenylenediamine (OPDA) as precursors. The obtained crystal structure, NMR, and mass data suggested the presence of 3-(1-benzimidazol-2-yl)propylphosphonium bromide (CHNPBr) as a molecular fluorophore (MF) on the surface of the TPP CNDs. The TPP CNDs showed better photostability than the commercially available MitoTracker™ Green and were highly capable for long-term imaging of mitochondrial fission during hyperglycemic conditions and structural changes upon an antidiabetic drug treatment, without altering their fluorescence nature.

摘要

在此，我们报告了新的红色发射、高稳定性和水溶性碳纳米点（TPP CNDs），可用于使用超分辨率径向波动（SRRF）显微镜可视化线粒体动力学。TPP CNDs 通过一步法合成，使用 3-（羧基丙基）三苯基溴化膦（TPP）和邻苯二胺（OPDA）作为前体。所得晶体结构、NMR 和质谱数据表明，[3-（1-苯并咪唑-2-基）丙基]（三苯基）溴化膦（CHNPBr）作为一种分子荧光团（MF）存在于 TPP CNDs 的表面。与市售的 MitoTracker™ Green 相比，TPP CNDs 具有更好的光稳定性，能够在高血糖条件下长时间成像线粒体裂变，并在抗糖尿病药物治疗时观察结构变化，而不会改变其荧光性质。