Thorne G M, Deneke C F, Gorbach S L
Infect Immun. 1979 Mar;23(3):690-9. doi: 10.1128/iai.23.3.690-699.1979.
Toxigenic strains of Escherichia coli isolated from humans were studied for adherence to human buccal mucosal epithelial cells. The E. coli strains were labeled with 3H-amino acids or fluorescein isothiocyanate. Toxigenic E. coli strains varied in their ability to adhere in the presence of mannose. Of 32 toxigenic strains examined, 52% bound to the buccal cells, whereas none of 8 control strains did so (Mann-Whitney U test, P =0.007). The control strains were nontoxigenic E. coli isolates from humans, enterotoxigenic E. coli isolates from animals, and E. coli K-12 containing the K88 or K99 plasmid; these strains exhibited only background-level adherence in this assay. Among the toxigenic E. coli strains that bound to human buccal mucosal cells, there was no correlation with mannose-resistant hemagglutination (MR-HA) of guinea pig and human erythrocytes. Screening 32 strains, we found the following phenotypes: (i) MR-HA+, buccal adherent; (ii) MR-HA+, buccal nonadherent; (iii) MR-HA-, buccal adherent. Presumably the third group represents strains with another type(s) of surface attachment components not involved in the MR-HA reaction. Our findings indicate that a number of bacterial surface structures can function in MR-HA and buccal adherence.
对从人类分离出的产毒大肠杆菌菌株进行了研究，以检测其对人类口腔黏膜上皮细胞的黏附情况。大肠杆菌菌株用³H-氨基酸或异硫氰酸荧光素进行标记。产毒大肠杆菌菌株在甘露糖存在下的黏附能力各不相同。在检测的32株产毒菌株中，52%与口腔细胞结合，而8株对照菌株均未结合（曼-惠特尼U检验，P = 0.007）。对照菌株包括从人类分离出的非产毒大肠杆菌、从动物分离出的产肠毒素大肠杆菌以及含有K88或K99质粒的大肠杆菌K-12；这些菌株在该检测中仅表现出背景水平的黏附。在与人类口腔黏膜细胞结合的产毒大肠杆菌菌株中，与豚鼠和人类红细胞的甘露糖抗性血凝反应（MR-HA）没有相关性。筛选32株菌株，我们发现了以下表型：（i）MR-HA阳性，口腔黏附；（ii）MR-HA阳性，口腔不黏附；（iii）MR-HA阴性，口腔黏附。推测第三组代表具有其他类型表面附着成分且不参与MR-HA反应的菌株。我们的研究结果表明，许多细菌表面结构可在MR-HA和口腔黏附中发挥作用。
