Ogawa T, Shimauchi H, Kusumoto Y, Hamada S
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
Immunology. 1990 Jan;69(1):8-13.
Bacteroides gingivalis fimbrial antigen incorporated into liposomes, but not in Tris-HCl buffer, significantly raised the levels of anti-fimbriae antibodies in serum, particularly of the IgG class, after oral primary and booster immunizations in BALB/c mice. An approximately linear relationship was observed between the dose of fimbrial antigen and the level of fimbriae-specific antibodies produced; antibody production reached its maximum at an immunization dosage of 500 micrograms of fimbriae per mouse. Fimbriae-specific antibody production was enhanced by use of a semi-synthetic adjuvant, a stearoyl derivative of sodium beta-N-acetylglucosaminyl-(1----4)-N-acetylmuramyl-L-alanyl-D-isoglutaminyl-(L) - stearoyl-(D)-meso-diamino-pimelic acid-(D)-amide-D-alanine (GM)-53) in liposomes. High anti-fimbriae antibody levels in serum and saliva were maintained for several months in the mice that had received two orally administered boosters of fimbrial antigen with GM-53 in liposomes. Salivary anti-fimbriae antibody levels, particularly of the IgA class, were markedly raised.
牙龈拟杆菌菌毛抗原包裹于脂质体中（而非Tris-HCl缓冲液中），在BALB/c小鼠经口服进行初次免疫和加强免疫后，能显著提高血清中抗菌毛抗体水平，尤其是IgG类抗体水平。观察到菌毛抗原剂量与所产生的菌毛特异性抗体水平之间存在近似线性关系；在每只小鼠免疫剂量为500微克菌毛时，抗体产生达到最大值。通过使用半合成佐剂——β-N-乙酰葡糖胺基-(1→4)-N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺基-(L)-硬脂酰-(D)-内消旋二氨基庚二酸-(D)-酰胺-D-丙氨酸（GM）-53的硬脂酰衍生物，脂质体中菌毛特异性抗体的产生得以增强。在接受两次口服脂质体中含GM-53的菌毛抗原加强免疫的小鼠中，血清和唾液中的高抗菌毛抗体水平维持了数月。唾液中抗菌毛抗体水平，尤其是IgA类抗体水平显著升高。
