Kundu Payel, Yasuhara Kanon, Brandes Mikah S, Zweig Jonathan A, Neff Cody J, Holden Sarah, Kessler Kat, Matsumoto Steven, Offner Halina, Waslo Carin Stewart, Vandenbark Arthur, Soumyanath Amala, Sherman Larry S, Raber Jacob, Gray Nora E, Spain Rebbeca Irene
Oregon Health & Science University.
Portland VA Medical Center.
Res Sq. 2023 Oct 6:rs.3.rs-3393042. doi: 10.21203/rs.3.rs-3393042/v1.
Centella asiatica (Centella) is a traditional botanical medicine that shows promise in treating dementia based on behavioral alterations seen in animal models of aging and cognitive dysfunction. In order to determine if Centella could similarly improve cognitive function and reduce disease burden in multiple sclerosis (MS), we tested its effects in the neuroinflammatory experimental autoimmune encephalomyelitis (EAE) model of MS. In two independent experiments, C57BL/6J mice were treated following induction of EAE with either a standardized water extract of Centella (CAW) or placebo for 2 weeks. At the dosing schedule and concentrations tested, CAW did not improve behavioral performance, EAE motor disability, or degrees of demyelination. However, CAW-treated mice demonstrated increases in nuclear factor (erythroid-derived 2)-like 2 and other antioxidant response element genes, and increases in mitochondrial respiratory activity. Caw also decreased spinal cord inflammation. Our findings indicate that CAW can increase antioxidant gene expression and mitochondrial respiratory activity in mice with EAE, supporting investigation of the clinical effects of CAW in people with MS.
积雪草是一种传统植物药，基于在衰老和认知功能障碍动物模型中观察到的行为改变，它在治疗痴呆方面显示出前景。为了确定积雪草是否同样能改善多发性硬化症（MS）患者的认知功能并减轻疾病负担，我们在MS的神经炎症性实验性自身免疫性脑脊髓炎（EAE）模型中测试了其效果。在两项独立实验中，C57BL/6J小鼠在EAE诱导后，用积雪草标准化水提取物（CAW）或安慰剂治疗2周。在所测试的给药方案和浓度下，CAW并未改善行为表现、EAE运动功能障碍或脱髓鞘程度。然而，经CAW治疗的小鼠显示出核因子（红细胞衍生2）样2和其他抗氧化反应元件基因增加，以及线粒体呼吸活性增加。CAW还减轻了脊髓炎症。我们的研究结果表明，CAW可增加EAE小鼠的抗氧化基因表达和线粒体呼吸活性，支持对CAW在MS患者中的临床效果进行研究。
