加味导赤散对复发性阿弗他溃疡大鼠 Th1/Th2 的影响。
The effect of Jiaweidaochi powder on Th1/Th2 in rats with recurrent aphthous ulcer.
机构信息
Changsha Stomatological Hospital, Changsha 410000, China.
Xiangya Stomatological Hospital, Central South University, Changsha 410000, China.
出版信息
Arch Oral Biol. 2023 Dec;156:105828. doi: 10.1016/j.archoralbio.2023.105828. Epub 2023 Oct 21.
OBJECTIVE
To elucidate the effect of Jiaweidaochi powder on the Th1/Th2 ratio in rats with recurrent aphthous ulcers (RAU).
DESIGN
40 Sprague-Dawley rats were included in this study, randomly divided into a control group, a model group, and three treatment groups (0.5 g/ ml, 1 g/ml, 2 g/ml Jiaweidaochi powder). The RAU model of rats was established by autoantigen injection. The effects of Jiaweidaochi powder on the expression of INFG, IL-4, TBX21, and GATA3 mRNA were detected by real-time PCR. The expression of IFN-γ, IL-4, and IFN-γ/IL-4 proteins in oral ulcer tissue and serum of rats were analyzed by Western blot and ELISA, respectively. The proportion of Th1 cells and Th2 cells in RAU rats was analyzed by flow cytometry.
RESULTS
Jiaweidaochi powder reduced the number, diameter, and duration of oral ulcers in RAU rats. Real-time PCR showed that middle and high-dose Jiaweidaochi powder decreased the expression of INFG TBX21 mRNA and increased the expression of IL-4 and GATA3 mRNA in the oral tissue of RAU rats. ELISA and western blot confirmed that the expression of IFN-γ protein was significantly decreased, and the level of IL-4 protein was increased both in oral tissue and serum of RAU rats treated with middle or high doses of Jiaweidaochi powder. Flow cytometry found that the Jiaweidaochi powder decreased the proportion of Th1 cells and increased the proportion of Th2 cells in RAU rats.
CONCLUSION
This study found that Jiaweidaochi powder promoted the balance of Th1/Th2 in RAU rats, contributing to the healing of RAU.
目的
阐明加味导赤散对复发性阿弗他溃疡（RAU）大鼠 Th1/Th2 比值的影响。
设计
将 40 只 Sprague-Dawley 大鼠纳入本研究，随机分为对照组、模型组和三个治疗组（0.5 g/ml、1 g/ml、2 g/ml 加味导赤散）。通过自体抗原注射建立大鼠 RAU 模型。实时 PCR 检测加味导赤散对 INFG、IL-4、TBX21 和 GATA3 mRNA 表达的影响。Western blot 和 ELISA 分别分析大鼠口腔组织和血清中 IFN-γ、IL-4 和 IFN-γ/IL-4 蛋白的表达，流式细胞术分析 RAU 大鼠 Th1 细胞和 Th2 细胞的比例。
结果
加味导赤散减少 RAU 大鼠口腔溃疡的数量、直径和持续时间。实时 PCR 显示，中、高剂量加味导赤散降低 RAU 大鼠口腔组织中 INFG、TBX21 mRNA 的表达，增加 IL-4 和 GATA3 mRNA 的表达。ELISA 和 western blot 证实，中、高剂量加味导赤散治疗后，RAU 大鼠口腔组织和血清中 IFN-γ 蛋白表达明显降低，IL-4 蛋白水平升高。流式细胞术发现加味导赤散降低了 RAU 大鼠 Th1 细胞的比例，增加了 Th2 细胞的比例。
结论
本研究发现加味导赤散促进 RAU 大鼠 Th1/Th2 平衡，有助于 RAU 的愈合。
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