Materials Sciences and Technology Division, CSIR North-East Institute of Science and Technology, Jorhat 785006, Assam, India.
Centre for Preclinical Studies, CSIR-North East Institute of Science and Technology, Jorhat 785006, Assam, India.
ACS Appl Mater Interfaces. 2024 Mar 27;16(12):14645-14660. doi: 10.1021/acsami.3c12108. Epub 2024 Mar 13.
The greater advantages and wide applications of zero-dimensional nanodots inspire researchers to develop new materials. Therefore, novel borophene quantum dots (QDs) were prepared by a hydrothermal liquid exfoliation technique using water medium. The borophene QDs proved to be highly stable in water medium for more than 120 days. The synthesized borophene QDs revealed intrinsic peroxidase mimetic activity using two chromogenic substrates, 3,3',5,5'-tetramethylbenzidine (TMB) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS). The excellent intrinsic peroxidase activity toward TMB and ABTS substrates was executed using optimal reaction conditions (pH, borophene QDs' concentration, incubation time, and temperature). The formation of hydroxyl radicals in the presence of HO upon TMB and ABTS oxidation played a significant role in the peroxidase reaction. The borophene QDs further proved to be successful for the colorimetric detection of antibiotics (oxytetracycline and tetracycline) using both TMB and ABTS peroxidase substrates. The limit of detection (LOD) for oxytetracycline and tetracycline was found to be 1.10 and 1.02 μM using TMB and 1.03 and 1.02 μM using ABTS chromogenic substrates, respectively. In addition, the fluorescence sensing of oxytetracycline and tetracycline over borophene QDs was also examined. The high fluorescence of borophene QDs (turn ON) was quenched (turn OFF) by oxytetracycline and tetracycline through the inner filter effect mechanism. The LOD of the fluorescence sensing of oxytetracycline and tetracycline was 1.14 and 1.08 μM, respectively. Interestingly, the borophene QDs could be used for the sensitive and selective colorimetric and fluorometric sensing of oxytetracycline and tetracycline after 120 days of storage. The synthesized borophene QDs with long-term stability and real sample analysis provide new insight as nanozymes with higher peroxidase mimetic and fluorescence performance and can be further exploited for medical diagnosis and environmental toxicants' detection.
零维纳米点的巨大优势和广泛应用激发了研究人员开发新材料。因此，本文采用水热液相剥离技术，以水为介质制备了新型硼烯量子点（QD）。硼烯 QD 在水中介质中可稳定存在超过 120 天。合成的硼烯 QD 对两种显色底物（3,3',5,5'-四甲基联苯胺（TMB）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS））表现出固有过氧化物酶模拟活性。在 TMB 和 ABTS 底物的最佳反应条件（pH、硼烯 QD 浓度、孵育时间和温度）下，硼烯 QD 对 TMB 和 ABTS 底物表现出优异的内在过氧化物酶活性。在 TMB 和 ABTS 氧化过程中，HO 存在时形成的羟基自由基在过氧化物酶反应中发挥了重要作用。硼烯 QD 还成功地用于使用 TMB 和 ABTS 过氧化物酶底物对抗生素（土霉素和四环素）进行比色检测。使用 TMB 和 ABTS 显色底物检测土霉素和四环素的检测限（LOD）分别为 1.10 和 1.02 μM。此外，还研究了硼烯 QD 对土霉素和四环素的荧光传感。土霉素和四环素通过内滤效应机制使硼烯 QD 的高荧光（开启）猝灭（关闭）。荧光传感土霉素和四环素的 LOD 分别为 1.14 和 1.08 μM。有趣的是，硼烯 QD 在经过 120 天储存后，仍可用于土霉素和四环素的灵敏、选择性比色和荧光传感。具有长期稳定性的合成硼烯 QD 及其对实际样品的分析为具有更高过氧化物酶模拟和荧光性能的纳米酶提供了新的见解，并可进一步用于医学诊断和环境毒物检测。
